Designing a conserved peptide-based subunit vaccine against SARS-CoV-2 using immunoinformatics approach.

Autor: Oladipo EK; Department of Microbiology, Laboratory of Molecular Biology, Immunology and Bioinformatics, Adeleke University, Ede, Osun State Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo State Nigeria., Ajayi AF; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State Nigeria., Onile OS; Biotechnology Programme, Department of Biological Sciences, Elizade University, Ilara-Mokin, Nigeria., Ariyo OE; Department of Medicine, Infectious Diseases and Tropical Medicine Unit, Federal Teaching Hospital, Ido-Ekiti, Ekiti State Nigeria., Jimah EM; Department of Medical Microbiology and Parasitology, University of Ilorin, Ilorin, Kwara State Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo State Nigeria., Ezediuno LO; Department of Microbiology and Parasitology, University of Ilorin, Ilorin, Kwara State Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo State Nigeria., Adebayo OI; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo State Nigeria., Adebayo ET; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo State Nigeria., Odeyemi AN; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo State Nigeria., Oyeleke MO; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State Nigeria., Oyewole MP; Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State Nigeria., Oguntomi AS; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo State Nigeria., Akindiya OE; Microbiology Programme, Department of Biological Science, Olusegun Agagu University of Science and Technology, Okitipupa, Ondo State Nigeria., Aremu VO; Reproduction and Bioinformatics Unit, Department of Medical Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State Nigeria.; Genomics Unit, Helix Biogen Consult, Ogbomoso, Oyo State Nigeria., Aboderin DO; Department of Medical Microbiology and Parasitology, University of Lagos, Lagos, Lagos State Nigeria., Oloke JK; Department of Natural Sciences, Precious Cornerstone University, Ibadan, Oyo State Nigeria.
Jazyk: angličtina
Zdroj: In silico pharmacology [In Silico Pharmacol] 2021 Jan 06; Vol. 9 (1), pp. 8. Date of Electronic Publication: 2021 Jan 06 (Print Publication: 2021).
DOI: 10.1007/s40203-020-00062-x
Abstrakt: The widespread of coronavirus (COVID-19) is a new global health crisis that poses a threat to the world. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) originated in bats and was discovered first in Wuhan, Hubei province, China in December 2019. Immunoinformatics and bioinformatics tools were employed for the construction of a multi-epitope subunit vaccine to prevent the diseases. The antigenicity, toxicity and allergenicity of all epitopes used in the construction of the vaccine were predicted and then conjugated with adjuvants and linkers. Vaccine Toll-Like Receptors (2, 3, 4, 8 and 9) complex was also evaluated. The vaccine construct was antigenic, non-toxic and non-allergic, which indicates the vaccines ability to induce antibodies in the host, making it an effective vaccine candidate.
Supplementary Information: The online version contains supplementary material available at 10.1007/s40203-020-00062-x.
Competing Interests: Conflict of interestThe authors declare no conflict of interest.
(© The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021.)
Databáze: MEDLINE
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