Two cyclin Bs are differentially modulated by glucose and sucrose during maize germination.

Autor: Lara-Núñez A; Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: auroraln@unam.mx., Romero-Sánchez DI; Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: dianqa03@gmail.com., Axosco-Marín J; Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: axoscomj@gmail.com., Garza-Aguilar SM; Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: qfbsaramargaritaga@gmail.com., Gómez-Martínez AE; Facultad de Química, Bioterio, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: gomatona@unam.mx., Ayub-Miranda MF; Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: qaferayub@gmail.com., Bravo-Alberto CE; Bio-Rad México, Eugenia 197, Int. Piso 10A. Col. Narvarte, Benito Juarez, C.P. 03020, CDMX, México. Electronic address: carloseverardobravo@gmail.com., Vázquez-Santana S; Facultad de Ciencias, Departamento de Biología Comparada, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: svs@ciencias.unam.mx., Vázquez-Ramos JM; Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: jorman@unam.mx.
Jazyk: angličtina
Zdroj: Biochimie [Biochimie] 2021 Mar; Vol. 182, pp. 108-119. Date of Electronic Publication: 2021 Jan 06.
DOI: 10.1016/j.biochi.2020.12.013
Abstrakt: Cell proliferation during seed germination is determinant for an appropriate seedling establishment. The present work aimed to evaluate the participation of two maize B-type Cyclins during germination and under the stimulus of two simple sugars: sucrose and glucose. We found out that the corresponding genes, ZmCycB1;2 and ZmCycB2;1, increased their expression at 24 h of germination, but only ZmCycB1;2 responded negatively to sugar type at the highest sugar concentration tested (120 mM). Also, CycB1;2 showed differential protein levels along germination in response to sugar, or its absence. Both CycBs interacted with CDKA;1 and CDKB1;1 by pull down assays. By an immunoprecipitation approach, it was found that each CycB associated with two CDKB isoforms (34 and 36 kDa). A higher proportion of CycB1;2-CDKB-36kDa was coincident to an increased kinase activity in the presence of sugar and particularly in glucose treatment at 36 h of imbibition. CycB1;2-CDKB activity increased in parallel to germination advance and this was dependent on sugar: glucose > sucrose > No sugar treatment. At RAM, CycB1;2 was more abundant in nuclei on Glucose at late germination; DNA-CycB1;2 colocalization was parallel to CycB1;2 inside the nucleus. Overall, results point out CycB1;2 as a player on promoting proliferation during germination by binding a specific CDKB isoform partner and changing its cellular localization to nuclei, co-localizing with DNA, being glucose a triggering signal.
Competing Interests: Declaration of competing interest None.
(Copyright © 2021 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
Databáze: MEDLINE
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