| Autor: |
Wani MR; Cytogenetics and Molecular Toxicology Laboratory, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India., Maheshwari N; Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India., Shadab G; Cytogenetics and Molecular Toxicology Laboratory, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India. gghas.amu@gmail.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Environmental science and pollution research international [Environ Sci Pollut Res Int] 2021 May; Vol. 28 (18), pp. 22664-22678. Date of Electronic Publication: 2021 Jan 09. |
| DOI: |
10.1007/s11356-020-12139-3 |
| Abstrakt: |
Titanium dioxide nanoparticles (TiO 2 NPs) are widely used in food, edible dyes, and other commercial products. Human exposure to TiO 2 NPs has raised concerns regarding their toxic potential. Various studies have evaluated the TiO 2 NPs-induced toxicity, oxidative damage to the cellular components, and genotoxicity. In the present study, we examined whether co-treatment with the dietary antioxidant eugenol can attenuate or protect against TiO 2 NPs-induced toxicity. We exposed the adult male Wistar rats to TiO 2 NPs (150 mg/kg body weight) by intraperitoneal injection (i.p.) either alone or as co-treatment with eugenol (1-10 mg/kg body weight) once a day for 14 days. The untreated rats were supplied saline and served as control. Titanium (Ti) accumulation in various tissues was analyzed by inductively coupled plasma mass spectrometry. Serum levels of liver and kidney biomarkers and oxidative stress markers in the liver, kidney, and spleen were determined. A significant increase in hydrogen peroxide level confirmed that oxidative stress occurred in these tissues. TiO 2 NPs induced oxidation of lipids, and decreased glutathione level and antioxidant enzyme activity in the kidney, liver, and spleen of treated rats. TiO 2 NPs also increased the serum levels of alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, albumin, and total cholesterol and decreased the blood urea nitrogen, uric acid, and total bilirubin in serum, which indicates oxidative damage to the liver and kidney. In eugenol and TiO 2 NPs co-treated rats, all these changes were mitigated. Single-cell gel electrophoresis (comet assay) of lymphocytes showed longer comet tail length in TiO 2 NPs-treated groups, indicating DNA damage while tail length was reduced in eugenol and TiO 2 NPs co-treated groups. Thus, it seems that eugenol can be used as a chemoprotective agent against TiO 2 NPs-induced toxicity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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