Synergistic Effect of Two Nanotechnologies Enhances the Protective Capacity of the Theileria parva Sporozoite p67C Antigen in Cattle.
| Autor: | Lacasta A; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya; a.lacasta@cgiar.org., Mody KT; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland 4072, Australia., De Goeyse I; Enzootic, Vector-borne and Bee Diseases, Sciensano, 1180 Brussels, Belgium.; Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium., Yu C; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland 4072, Australia., Zhang J; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland 4072, Australia., Nyagwange J; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya., Mwalimu S; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya., Awino E; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya., Saya R; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya., Njoroge T; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya., Muriuki R; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya., Ndiwa N; Research Methods Group, International Livestock Research Institute, Nairobi 00100, Kenya; and., Poole EJ; Research Methods Group, International Livestock Research Institute, Nairobi 00100, Kenya; and., Zhang B; Department of Agriculture and Fisheries, Brisbane, Queensland 4102, Australia., Cavallaro A; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland 4072, Australia., Mahony TJ; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland 4072, Australia., Steinaa L; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya., Mitter N; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland 4072, Australia., Nene V; Animal and Human Health Program, International Livestock Research Institute, Nairobi 00100, Kenya. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2021 Feb 15; Vol. 206 (4), pp. 686-699. Date of Electronic Publication: 2021 Jan 08. |
| DOI: | 10.4049/jimmunol.2000442 |
| Abstrakt: | East Coast fever (ECF), caused by Theileria parva , is the most important tick-borne disease of cattle in sub-Saharan Africa. Practical disadvantages associated with the currently used live-parasite vaccine could be overcome by subunit vaccines. An 80-aa polypeptide derived from the C-terminal portion of p67, a sporozoite surface Ag and target of neutralizing Abs, was the focus of the efforts on subunit vaccines against ECF and subjected to several vaccine trials with very promising results. However, the vaccination regimen was far from optimized, involving three inoculations of 450 μg of soluble p67C (s-p67C) Ag formulated in the Seppic adjuvant Montanide ISA 206 VG. Hence, an improved formulation of this polypeptide Ag is needed. In this study, we report on two nanotechnologies that enhance the bovine immune responses to p67C. Individually, HBcAg-p67C (chimeric hepatitis B core Ag virus-like particles displaying p67C) and silica vesicle (SV)-p67C (s-p67C adsorbed to SV-140-C (Copyright © 2021 The Authors.) |
| Databáze: | MEDLINE |
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