| Autor: |
Ježić M; University of Zagreb, Faculty of Science, Department of Biology, Division of Microbiology, Zagreb, Croatia., Schwarz JM; Swiss Federal Research Institute WSL, 8903 Birmensdorf, Switzerland., Prospero S; Swiss Federal Research Institute WSL, 8903 Birmensdorf, Switzerland., Sotirovski K; Ss Cyril and Methodius University in Skopje, Hans Em Faculty of Forest Sciences, Landscape Architecture and Environmental Engineering, 1000 Skopje, North Macedonia., Risteski M; Ss Cyril and Methodius University in Skopje, Hans Em Faculty of Forest Sciences, Landscape Architecture and Environmental Engineering, 1000 Skopje, North Macedonia., Ćurković-Perica M; University of Zagreb, Faculty of Science, Department of Biology, Division of Microbiology, Zagreb, Croatia., Nuskern L; University of Zagreb, Faculty of Science, Department of Biology, Division of Microbiology, Zagreb, Croatia., Krstin L; University J. J. Strossmayer of Osijek, Department of Biology, 31000 Osijek, Croatia., Katanić Z; University J. J. Strossmayer of Osijek, Department of Biology, 31000 Osijek, Croatia., Maleničić E; University of Zagreb, Faculty of Science, Department of Biology, Division of Microbiology, Zagreb, Croatia., Poljak I; University of Zagreb, Faculty of Forestry and Wood Technology, Department of Forest Genetics, Dendrology and Botany, 10000 Zagreb, Croatia., Idžojtić M; University of Zagreb, Faculty of Forestry and Wood Technology, Department of Forest Genetics, Dendrology and Botany, 10000 Zagreb, Croatia., Rigling D; Swiss Federal Research Institute WSL, 8903 Birmensdorf, Switzerland. |
| Abstrakt: |
Chestnut blight has spread throughout Europe since the introduction of its causal agent, Cryphonectria parasitica , >70 years ago. In our study, we analyzed the diversity of vegetative compatibility (vc) and microsatellite genotypes of C. parasitica , as well as sequence diversity of Cryphonectria hypovirus 1 (CHV1) in six populations from Switzerland, Croatia, and North Macedonia. Resampling of local populations that were already investigated more than a decade ago allowed us to analyze the spatial and temporal population structure across an invasive range of the pathogen in Europe. Regardless of which genetic marker was used, the >60-year-old Swiss and Croatian populations had high population diversity, whereas more recent North Macedonian populations were mostly clonal. These diversity differences between the investigated populations remained stable over time. A high diversity of CHV1 was observed in all three countries, with North Macedonian strains forming a separate cluster from strains obtained in other countries. No correlation between vc diversity and CHV1 prevalence was observed, suggesting a well-established and maintained natural hypovirulence in all countries, further corroborated by an observed increase in genetic diversity of Croatian C. parasitica populations over time, without collapse of CHV1 prevalence. |
