| Autor: |
Kilimci U; Chemistry Division, Faculty of Science and Arts, Adnan Menderes University, Aydın, Turkey., Evli S; Chemistry Division, Faculty of Science and Arts, Adnan Menderes University, Aydın, Turkey., Öndeş B; Chemistry Division, Faculty of Science and Arts, Adnan Menderes University, Aydın, Turkey., Uygun M; Chemistry Division, Faculty of Science and Arts, Adnan Menderes University, Aydın, Turkey., Uygun DA; Chemistry Division, Faculty of Science and Arts, Adnan Menderes University, Aydın, Turkey. daktasuygun@gmail.com. |
| Abstrakt: |
In this presented paper, concanavalin A-modified cysteine-functionalized Fe 3 O 4 /Ag core/shell magnetic nanoparticles were synthesized and used as a support material for inulinase enzyme, which has been intensively used for the preparation of high-fructose syrup by hydrolyzing inulin. Inulinase adsorption capacity of Con A-functionalized Ag-coated magnetic nanoparticles was optimized by changing medium pH, temperature, and initial inulinase concentration, and maximum inulinase adsorption capacity was found to be 655.32 mg/g nanoparticle by using 1.00 mg/mL of inulinase solution in pH 3.0 buffer system at 25 °C. Finally, efficient inulin degradation capacity of the inulinase immobilized magnetic nanoparticles was demonstrated by TLC studies and released fructose amount was determined as 0.533 mg/mL only within the 5 min of hydrolysis. This newly developed hydrolysis strategy holds considerable promise to produce high-fructose syrup in many industries. |
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