Autor: |
Vicente TFL; Departamento de Biologia, CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal., Gonçalves MFM; Departamento de Biologia, CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal., Brandão C; Departamento de Biologia, CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal., Fidalgo C; Departamento de Biologia, CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal., Alves A; Departamento de Biologia, CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal. |
Abstrakt: |
Fungal communities associated with macroalgae remain largely unexplored. To characterize algicolous fungal communities using culture dependent methods, macroalgae were collected from different sampling sites in the Ria de Aveiro estuary, Portugal. From a collection of 486 isolates that were obtained, 213 representative isolates were selected through microsatellite-primed PCR (MSP-PCR) fingerprinting analysis. The collection yielded 33 different genera, which were identified using the ITS region of the rDNA. The results revealed that the most abundant taxa in all collections were Acremonium -like species: Alternaria , Cladosporium , Leptobacillium and Penicillium . The fungal community composition varied with macroalgae species. Through multilocus phylogenetic analyses based on ITS, tub2 , tef1-α and actA sequences, in addition to detailed morphological data, we propose Cladosporium rubrum sp. nov. (type strain=CMG 28=MUM 19.39) and Hypoxylon aveirense sp. nov. (type strain=CMG 29=MUM 19.40) as novel species. |