Acquisition of multidrug-resistant bacteria and encoding genes among French pilgrims during the 2017 and 2018 Hajj.

Autor: Hoang VT; IRD, AP-HM, SSA, VITROME, Aix Marseille University, Marseille, France.; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.; Thai Binh University of Medicine and Pharmacy, Thai Binh, Vietnam., Dao TL; IRD, AP-HM, SSA, VITROME, Aix Marseille University, Marseille, France.; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.; Thai Binh University of Medicine and Pharmacy, Thai Binh, Vietnam., Ly TDA; IRD, AP-HM, SSA, VITROME, Aix Marseille University, Marseille, France.; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France., Gouriet F; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.; IRD, AP-HM, MEPHI, Aix Marseille University, Marseille, France., Hadjadj L; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.; IRD, AP-HM, MEPHI, Aix Marseille University, Marseille, France., Belhouchat K; IRD, AP-HM, SSA, VITROME, Aix Marseille University, Marseille, France.; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France., Chaht KL; IRD, AP-HM, SSA, VITROME, Aix Marseille University, Marseille, France.; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France., Yezli S; The Global Centre for Mass Gatherings Medicine, Ministry of Health, Riyadh, Saudi Arabia., Alotaibi B; The Global Centre for Mass Gatherings Medicine, Ministry of Health, Riyadh, Saudi Arabia., Raoult D; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.; IRD, AP-HM, MEPHI, Aix Marseille University, Marseille, France., Parola P; IRD, AP-HM, SSA, VITROME, Aix Marseille University, Marseille, France.; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France., Baron SA; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.; IRD, AP-HM, MEPHI, Aix Marseille University, Marseille, France., de Santi VP; IRD, AP-HM, SSA, VITROME, Aix Marseille University, Marseille, France.; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.; French Military Center for Epidemiology and Public Health Marseille, Marseille, France., Rolain JM; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.; IRD, AP-HM, MEPHI, Aix Marseille University, Marseille, France., Gautret P; IRD, AP-HM, SSA, VITROME, Aix Marseille University, Marseille, France. philippe.gautret@club-internet.fr.; Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France. philippe.gautret@club-internet.fr.
Jazyk: angličtina
Zdroj: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2021 Jun; Vol. 40 (6), pp. 1199-1207. Date of Electronic Publication: 2021 Jan 07.
DOI: 10.1007/s10096-020-04122-0
Abstrakt: The objective of this study is to determine the acquisition of multidrug-resistant (MDR) bacteria and antibiotic resistance-encoding genes by French Hajj pilgrims and associated risk factors. Pilgrims traveling during the 2017 and 2018 Hajj were recruited. All pilgrims underwent two successive systematic nasopharyngeal and rectal swabs, pre- and post-Hajj. Specific culture media were used to screen for MDR bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant bacteria, and extended spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E). qPCR was used to identify antibiotic resistance-encoding genes from cultured isolates. Direct screening of genes encoding for colistin resistance (mcr-1, 2, 3, 4, 5, and 8) from nasopharyngeal and rectal swabs was performed using qPCR, and positive qPCR results were simultaneously tested by sequencing. There were 268 pilgrims included. The percentage of pilgrims acquiring MDR bacteria during the Hajj was 19.4%. A total of 81 strains were isolated (1 carbapenem-resistant Acinetobacter baumannii, 12 MRSA, and 68 ESBL-E). ESBL-E strains were found in rectal samples of 6.0% pilgrims pre-Hajj and of 16.4% pilgrims post-Hajj. Only 0.4% pilgrims were positive for CARB post-Hajj and 1.9% carried nasal MRSA pre- and post-Hajj. In addition, 23 (8.6%) post-Hajj rectal swabs were positive for mcr genes (19 mcr-1 gene and 4 mcr-4 gene). No significant association was found between co-factors and acquisition of MDR bacteria or mcr genes. MDR bacteria and genes are acquired by pilgrims during the Hajj mass gathering. Rationalization of antibiotic consumption and implementation of measures to prevent transmission of bacteria among pilgrims during the event are of paramount importance.
Databáze: MEDLINE
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