Miniaturized modular-array fluorescence microscopy.
| Autor: | Son J; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA., Mandracchia B; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA., Jia S; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Biomedical optics express [Biomed Opt Express] 2020 Nov 18; Vol. 11 (12), pp. 7221-7235. Date of Electronic Publication: 2020 Nov 18 (Print Publication: 2020). |
| DOI: | 10.1364/BOE.410605 |
| Abstrakt: | Fluorescence live-cell imaging allows for continuous interrogation of cellular behaviors, and the recent development of portable live-cell imaging platforms has rapidly transformed conventional schemes with high adaptability, cost-effective functionalities and easy accessibility to cell-based assays. However, broader applications remain restrictive due to compatibility with conventional cell culture workflow and biochemical sensors, accessibility to up-right physiological imaging, or parallelization of data acquisition. Here, we introduce miniaturized modular-array fluorescence microscopy (MAM) for compact live-cell imaging in flexible formats. We advance the current miniscopy technology to devise an up-right modular architecture, each combining a gradient-index (GRIN) objective and individually-addressed illumination and acquisition components. Parallelization of an array of such modular devices allows for multi-site data acquisition in situ using conventional off-the-shelf cell chambers. Compared with existing methods, the device offers a high fluorescence sensitivity and efficiency, exquisite spatiotemporal resolution (∼3 µm and up to 60 Hz), a configuration compatible with conventional cell culture assays and physiological imaging, and an effective parallelization of data acquisition. The system has been demonstrated using various calibration and biological samples and experimental conditions, representing a promising solution to time-lapse in situ single-cell imaging and analysis. Competing Interests: The authors declare that there are no conflicts of interest to this article. (© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|