Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface.

Autor: Makhulu EE; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; Biochemistry and Molecular Biology Department, Egerton University, Nakuru, Kenya., Villinger J; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya., Adunga VO; Biochemistry and Molecular Biology Department, Egerton University, Nakuru, Kenya., Jeneby MM; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; Institute of Primate Research, National Museums of Kenya, Nairobi, Kenya., Kimathi EM; Department of Medical Biochemistry, Kisii University, Kisii, Kenya., Mararo E; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; The Roslin Institute, Easter Bush Campus, University of Edinburgh, Midlothian City, Scotland., Oundo JW; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; School of Biological Sciences, University of Nairobi, Nairobi, Kenya., Musa AA; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; Department of Medical Laboratory Sciences, Kenyatta University, Nairobi, Kenya., Wambua L; International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.; School of Biological Sciences, University of Nairobi, Nairobi, Kenya.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2021 Jan 06; Vol. 15 (1), pp. e0008267. Date of Electronic Publication: 2021 Jan 06 (Print Publication: 2021).
DOI: 10.1371/journal.pntd.0008267
Abstrakt: African trypanosomiasis (AT) is a neglected disease of both humans and animals caused by Trypanosoma parasites, which are transmitted by obligate hematophagous tsetse flies (Glossina spp.). Knowledge on tsetse fly vertebrate hosts and the influence of tsetse endosymbionts on trypanosome presence, especially in wildlife-human-livestock interfaces, is limited. We identified tsetse species, their blood-meal sources, and correlations between endosymbionts and trypanosome presence in tsetse flies from the trypanosome-endemic Maasai Mara National Reserve (MMNR) in Kenya. Among 1167 tsetse flies (1136 Glossina pallidipes, 31 Glossina swynnertoni) collected from 10 sampling sites, 28 (2.4%) were positive by PCR for trypanosome DNA, most (17/28) being of Trypanosoma vivax species. Blood-meal analyses based on high-resolution melting analysis of vertebrate cytochrome c oxidase 1 and cytochrome b gene PCR products (n = 354) identified humans as the most common vertebrate host (37%), followed by hippopotamus (29.1%), African buffalo (26.3%), elephant (3.39%), and giraffe (0.84%). Flies positive for trypanosome DNA had fed on hippopotamus and buffalo. Tsetse flies were more likely to be positive for trypanosomes if they had the Sodalis glossinidius endosymbiont (P = 0.0002). These findings point to complex interactions of tsetse flies with trypanosomes, endosymbionts, and diverse vertebrate hosts in wildlife ecosystems such as in the MMNR, which should be considered in control programs. These interactions may contribute to the maintenance of tsetse populations and/or persistent circulation of African trypanosomes. Although the African buffalo is a key reservoir of AT, the higher proportion of hippopotamus blood-meals in flies with trypanosome DNA indicates that other wildlife species may be important in AT transmission. No trypanosomes associated with human disease were identified, but the high proportion of human blood-meals identified are indicative of human African trypanosomiasis risk. Our results add to existing data suggesting that Sodalis endosymbionts are associated with increased trypanosome presence in tsetse flies.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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