A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells.

Autor:	Khazim M; Department of Engineering Mathematics, University of Bristol, Bristol, UK.; School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK.; BrisSynBio, Bristol, UK., Pedone E; Department of Engineering Mathematics, University of Bristol, Bristol, UK.; School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK.; BrisSynBio, Bristol, UK., Postiglione L; Department of Engineering Mathematics, University of Bristol, Bristol, UK.; School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK.; BrisSynBio, Bristol, UK., di Bernardo D; Telethon Institute of Genetics and Medicine, Pozzuoli (NA), Italy., Marucci L; Department of Engineering Mathematics, University of Bristol, Bristol, UK. lucia.marucci@bristol.ac.uk.; School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK. lucia.marucci@bristol.ac.uk.; BrisSynBio, Bristol, UK. lucia.marucci@bristol.ac.uk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2229, pp. 205-219.
DOI:	10.1007/978-1-0716-1032-9_10
Abstrakt:	Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.
Databáze:	MEDLINE
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