| Autor: |
Abu Almaaty AH; Zoology Department, Faculty of Science, Port Said University, Port Said, Egypt., Mosaad RM; Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt., Hassan MK; Zoology Department, Faculty of Science, Port Said University, Port Said, Egypt., Ali EHA; Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt., Mahmoud GA; Zoology Department, Faculty of Science, Port Said University, Port Said, Egypt., Ahmed H; Department of Physiology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt., Anber N; Emergency Hospital, Mansoura University, Mansoura, Egypt., Alkahtani S; Department of Zoology, Science College, King Saud University, Riyadh, 11451, Saudi Arabia., Abdel-Daim MM; Department of Zoology, Science College, King Saud University, Riyadh, 11451, Saudi Arabia.; Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt., Aleya L; Laboratoire Chrono-Environment, CNRS 6249, Université de Bourgogne Franche-Comté, Besançon, France., Hammad S; Department of Forensic Medicine and Veterinary Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt. Seddik.hammad@vet.svu.edu.eg. |
| Abstrakt: |
Alzheimer's disease (AD) is characterized by alterations in monoamines, oxidative stress, and metabolic dysfunctions. We aim to assess the therapeutic impacts of roots or leaf extract from Urtica dioica (UD; stinging nettle) against scopolamine (SCOP)-induced memory dysfunction, amnesia, and oxidative stress in rats. Spatial memory was assessed by Y maze test. Tissue analyses of norepinephrine (NE), dopamine (DA), serotonin (5-HT), malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH, GSSG), AMP, ADP, and ATP were assessed by HPLC. mRNA levels of Tau and Hsp70 were estimated by PCR. UD extracts particularly nettle root (NR) significantly normalized the SCOP-induced memory deficits even more potent than sermion (SR) and donepezil (DON). Similarly, NR had potent therapeutic impacts on the levels of cortical and hippocampal monoamines e.g. DA, NE, and 5-HT. SCOP induced a dramatic oxidative stress as measured by MDA, NO, and GSSG levels; however, UD extracts showed significant anti-oxidative stress impacts. Additionally, UD extracts restored ATP levels and reduced the levels of AMP and ADP compared to SCOP-treated rats. Furthermore, cortical Tau and hippocampal Hsp70 were modulated by UD extracts particularly NR compared to the SCOP group. In conclusion, UD extracts particularly roots have potential therapeutic impacts against SCOP-induced neuroinflammatory and/or Alzheimer-like phenotype in rats. |
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