Usage of atomic force microscopy for detection of the damaging effect of CdCl 2 on red blood cells membrane.

Autor: Demchenkov EL; FreezeM Inc, Nachshonim, Israel., Nagdalian AA; North Caucasus Federal University, Stavropol, Russian Federation. Electronic address: geniando@yandex.ru., Budkevich RO; North Caucasus Federal University, Stavropol, Russian Federation., Oboturova NP; North Caucasus Federal University, Stavropol, Russian Federation., Okolelova AI; Kuban State Agrarian University, Krasnodar, Russian Federation.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2021 Jan 15; Vol. 208, pp. 111683. Date of Electronic Publication: 2020 Nov 21.
DOI: 10.1016/j.ecoenv.2020.111683
Abstrakt: The possibility of detecting the damaging effect of cadmium salts on red blood cells (RBC) membrane by atomic force microscopy and light microscopy was studied. White wistar rats RBC were incubated with cadmium chloride in concentrations of 1 μg/l, 10 μg/l, 100 μg/l, and 1000 μg/l for the research. A comparison of sample preparation methods proposed by other authors in previous studies is made. The optimal method that does not significantly affect the change in the morphological features of the cell is selected. The quantitative assessment of damaged and destroyed RBC depending on the concentration of cadmium was performed by optical microscopy. The study showed that CdCl 2 has a damaging effect on the RBC membrane, which leads to the formation of non-specific cell forms. A comparative assessment was made between the methods of optical microscopy and atomic force microscopy for the suitability of studying the morphological characteristics of abnormal forms of the RBC. It is shown that the method of atomic force microscopy allows registering morphological changes in the RBC that cannot be registered by optical microscopy. It is pointed that CdCl 2 has effect on destruction of the RBC and the formation of specific bulges on the RBC membrane. Influence of CdCl 2 on the RBC mechanical properties was studied using atomic force microscopy. The possibility of using atomic force microscopy in studies of morphology and mechanical properties of the RBC under toxicity effect of cadmium is shown.
(Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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