| Autor: |
Chandrasekaran AR; The RNA Institute, University at Albany, State University of New York, New York, New York 12222, United States., MacIsaac M; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, United States.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States.; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115, United States., Vilcapoma J; The RNA Institute, University at Albany, State University of New York, New York, New York 12222, United States., Hansen CH; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, United States.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States.; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115, United States., Yang D; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, United States.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States.; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115, United States., Wong WP; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, United States.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States.; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115, United States., Halvorsen K; The RNA Institute, University at Albany, State University of New York, New York, New York 12222, United States. |
| Abstrakt: |
Molecular biomarkers play a key role in the clinic, aiding in diagnostics and prognostics, and in the research laboratory, contributing to our basic understanding of diseases. Detecting multiple and diverse molecular biomarkers within a single accessible assay would have great utility, providing a more comprehensive picture for clinical evaluation and research, but is a challenge with standard methods. Here, we report programmable DNA nanoswitches for multiplexed detection of up to 6 biomarkers at once with each combination of biomarkers producing a unique barcode signature among 64 possibilities. As a defining feature of our method, we show "mixed multiplexing" for simultaneous barcoded detection of different types of biomolecules, for example, DNA, RNA, antibody, and protein in a single assay. To demonstrate clinical potential, we show multiplexed detection of a prostate cancer biomarker panel in serum that includes two microRNA sequences and prostate specific antigen. |
