Targeting Uric Acid Prevents Brain Injury and Anxiety in a Rat Model of Hemorrhagic Shock.

Autor:	L'Ecuyer S; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada.; Research Center, University of Montreal Hospital Center University of Montreal Hospital Research Center (CRCHUM), Montréal, Canada., Gilbert K; Montreal Sacré-Coeur Hospital, Montréal, Canada., Brochu B; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada., Beyrouthy J; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada., Liu C; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada., Bouchard C; Montreal Sacré-Coeur Hospital, Montréal, Canada., Gagné MA; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada.; Montreal Sacré-Coeur Hospital, Montréal, Canada., Khazoom F; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada., Bernard F; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada.; Montreal Sacré-Coeur Hospital, Montréal, Canada., Rousseau G; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada.; Montreal Sacré-Coeur Hospital, Montréal, Canada., Charbonney E; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Canada.; Research Center, University of Montreal Hospital Center University of Montreal Hospital Research Center (CRCHUM), Montréal, Canada.; Montreal Sacré-Coeur Hospital, Montréal, Canada.
Jazyk:	angličtina
Zdroj:	Shock (Augusta, Ga.) [Shock] 2021 Aug 01; Vol. 56 (2), pp. 298-307.
DOI:	10.1097/SHK.0000000000001708
Abstrakt:	Abstract: Secondary brain injury following hemorrhagic shock (HS) is a frequent complication in patients, even in the absence of direct brain trauma, leading to behavioral changes and more specifically anxiety and depression. Despite preclinical studies showing inflammation and apoptosis in the brain after HS, none have addressed the impact of circulating mediators. Our group demonstrated an increased uric acid (UA) circulation in rats following HS. Since UA is implicated in endothelial dysfunction and inflammatory response, we hypothesized UA could alter the blood-brain barrier (BBB) and impact the brain. Male Wistar rats were randomly assigned to: SHAM, HS (hemorrhagic shock) and HS + U (hemorrhagic shock + 1.5 mg/kg of uricase). The uricase intervention, specifically targeting UA, was administered during fluid resuscitation. It prevented BBB dysfunction (fluorescein sodium salt permeability and expression of intercellular adhesion molecule-1) following HS. As for neuroinflammation, all of the results obtained (MPO activity; Iba1 and GFAP expression) showed a significant increase after HS, also prevented by the uricase. The same pattern was observed after quantification of apoptosis (caspase-3 activity and TUNEL) and neurodegeneration (Fluoro-Jade). Finally, the forced swim, elevated plus maze, and social interaction tests detected anxiety-like behavior after HS, which was blunted in rats treated with the uricase. In conclusion, we have identified UA as a new circulatory inflammatory mediator, responsible for brain alterations and anxious behavior after HS in a murine model. The ability to target UA holds the potential of an adjunctive therapeutic solution to reduce brain dysfunction related to hemorrhagic shock in human. Competing Interests: The authors report no conflicts of interest. (Copyright © 2020 by the Shock Society.)
Databáze:	MEDLINE
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