Single-Cell Technologies to Understand the Mechanisms of Cellular Adaptation in Chemostats.
| Autor: | Wright NR; Novo Nordisk A/S, Bagsvaerd, Denmark.; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark., Rønnest NP; Novo Nordisk A/S, Bagsvaerd, Denmark., Sonnenschein N; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2020 Dec 18; Vol. 8, pp. 579841. Date of Electronic Publication: 2020 Dec 18 (Print Publication: 2020). |
| DOI: | 10.3389/fbioe.2020.579841 |
| Abstrakt: | There is a growing interest in continuous manufacturing within the bioprocessing community. In this context, the chemostat process is an important unit operation. The current application of chemostat processes in industry is limited although many high yielding processes are reported in literature. In order to reach the full potential of the chemostat in continuous manufacture, the output should be constant. However, adaptation is often observed resulting in changed productivities over time. The observed adaptation can be coupled to the selective pressure of the nutrient-limited environment in the chemostat. We argue that population heterogeneity should be taken into account when studying adaptation in the chemostat. We propose to investigate adaptation at the single-cell level and discuss the potential of different single-cell technologies, which could be used to increase the understanding of the phenomena. Currently, none of the discussed single-cell technologies fulfill all our criteria but in combination they may reveal important information, which can be used to understand and potentially control the adaptation. Competing Interests: NW and NR were employed by the company Novo Nordisk A/S. The remaining author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2020 Wright, Rønnest and Sonnenschein.) |
| Databáze: | MEDLINE |
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