Generation of a Genetically Modified Chimeric Plasmodium falciparum Parasite Expressing Plasmodium vivax Circumsporozoite Protein for Malaria Vaccine Development.

Autor:	Miyazaki Y; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Marin-Mogollon C; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Imai T; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands.; Department of Infectious Diseases and Host Defense, Gunma University Graduate School of Medicine, Maebashi, Japan., Mendes AM; Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal., van der Laak R; TropIQ Health Sciences, Nijmegen, Netherlands., Sturm A; TropIQ Health Sciences, Nijmegen, Netherlands., Geurten FJA; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Miyazaki S; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Chevalley-Maurel S; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Ramesar J; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Kolli SK; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Kroeze H; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., van Schuijlenburg R; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Salman AM; The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom., Wilder BK; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Portland, Oregon, United States., Reyes-Sandoval A; The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom., Dechering KJ; TropIQ Health Sciences, Nijmegen, Netherlands., Prudêncio M; Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal., Janse CJ; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Khan SM; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Franke-Fayard B; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2020 Dec 17; Vol. 10, pp. 591046. Date of Electronic Publication: 2020 Dec 17 (Print Publication: 2020).
DOI:	10.3389/fcimb.2020.591046
Abstrakt:	Chimeric rodent malaria parasites with the endogenous circumsporozoite protein ( csp ) gene replaced with csp from the human parasites Plasmodium falciparum ( Pf ) and P. vivax ( Pv ) are used in preclinical evaluation of CSP vaccines. Chimeric rodent parasites expressing Pf CSP have also been assessed as whole sporozoite (WSP) vaccines. Comparable chimeric P. falciparum parasites expressing CSP of P. vivax could be used both for clinical evaluation of vaccines targeting Pv CSP in controlled human P. falciparum infections and in WSP vaccines targeting P. vivax and P. falciparum . We generated chimeric P. falciparum parasites expressing both Pf CSP and Pv CSP. These Pf - Pv CSP parasites produced sporozoite comparable to wild type P. falciparum parasites and expressed Pf CSP and Pv CSP on the sporozoite surface. Pf - Pv CSP sporozoites infected human hepatocytes and induced antibodies to the repeats of both Pf CSP and Pv CSP after immunization of mice. These results support the use of Pf - Pv CSP sporozoites in studies optimizing vaccines targeting Pv CSP. Competing Interests: KD holds stock in TropIQ Health Sciences. RL, AS and KD were employed by TropIQ Health Sciences. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2020 Miyazaki, Marin-Mogollon, Imai, Mendes, van der Laak, Sturm, Geurten, Miyazaki, Chevalley-Maurel, Ramesar, Kolli, Kroeze, van Schuijlenburg, Salman, Wilder, Reyes-Sandoval, Dechering, Prudêncio, Janse, Khan and Franke-Fayard.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu