Performance of at-home self-collected saliva and nasal-oropharyngeal swabs in the surveillance of COVID-19.

Autor: Braz-Silva PH; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil.; Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil., Mamana AC; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil., Romano CM; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil., Felix AC; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil., de Paula AV; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil., Fereira NE; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil., Buss LF; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil., Tozetto-Mendoza TR; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil., Caixeta RAV; Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil., Leal FE; Department of Medicine, Municipal University of São Caetano do Sul, São Caetano Do Sul, Brazil., Grespan RMZ; Department of Medicine, Municipal University of São Caetano do Sul, São Caetano Do Sul, Brazil., Bizário JCS; Department of Medicine, Municipal University of São Caetano do Sul, São Caetano Do Sul, Brazil., Ferraz ABC; Department of Medicine, Municipal University of São Caetano do Sul, São Caetano Do Sul, Brazil., Sapkota D; Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway., Giannecchini S; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., To KK; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li KaShing Faculty of Medicine of the University of Hong Kong, Special Administrative Region, China.; Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.; Department of Clinical Microbiology and Infection Control, The University of Hong Kong - Shenzhen Hospital, Shenzhen, China., Doglio A; Laboratory MICORALIS (Microbiologie Orale, Immunité Et Santé) School of Dentistry, University of Côte d'Azur, Nice, France., Mendes-Correa MC; Laboratory of Virology (LIM-52), Institute of Tropical Medicine of São Paulo, School of Medicine, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of oral microbiology [J Oral Microbiol] 2020 Dec 09; Vol. 13 (1), pp. 1858002. Date of Electronic Publication: 2020 Dec 09.
DOI: 10.1080/20002297.2020.1858002
Abstrakt: Background : SARS-CoV-2 quickly spreads in the worldwide population, imposing social restrictions to control the infection, being the massive testing another essential strategy to break the chain of transmission. Aim : To compare the performance of at-home self-collected samples - saliva and combined nasal-oropharyngeal swabs (NOP) - for SARS-CoV-2 detection in a telemedicine platform for COVID-19 surveillance. Material and methods : We analyzed 201 patients who met the criteria of suspected COVID-19. NOP sampling was combined (nostrils and oropharynx) and saliva collected using a cotton pad device. Detection of SARS-COV-2 was performed by using the Altona RealStar® SARS-CoV-2 RT-PCR Kit 1.0.  Results: There was an overall significant agreement (κ coefficient value of 0.58) between saliva and NOP. Considering results in either sample, 70 patients positive for SARS-CoV-2 were identified, with 52/70 being positive in NOP and 55/70 in saliva. This corresponds to sensitivities of 74.2% (95% CI; 63.7% to 83.1%) for NOP and 78.6% (95% CI; 67.6% to 86.6%) for saliva. Conclusion : Our data show the feasibility of using at-home self-collected samples (especially saliva), as an adequate alternative for SARS-CoV-2 detection. This new approach of testing can be useful to develop strategies for COVID-19 surveillance and for guiding public health decisions.
Competing Interests: No potential conflict of interest was reported by the authors.
(© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)
Databáze: MEDLINE
Externí odkaz: