Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study.

Autor: Berbéri A; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Lebanese University, Rafic Hariri Campus, POBox 5208-116, Beirut, Lebanon. aberberi@ul.edu.lb., Sabbagh J; Department of Restorative Dentistry and Endodontics, Faculty of Dental Medicine, Lebanese University, Beirut, Lebanon., Bou Assaf R; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Lebanese University, Rafic Hariri Campus, POBox 5208-116, Beirut, Lebanon., Ghassibe-Sabbagh M; Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, Beirut, Lebanon., Al-Nemer F; Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences-I, Lebanese University, Hadath, Beirut, Lebanon., El Majzoub R; School of Pharmacy (Department of Biomedical Sciences), Lebanese International University, Mazraa, 146404, Lebanon., Fayyad-Kazan M; Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, Beirut, Lebanon. mfayyadk@gmail.com.; Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences-I, Lebanese University, Hadath, Beirut, Lebanon. mfayyadk@gmail.com., Badran B; Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences-I, Lebanese University, Hadath, Beirut, Lebanon.
Jazyk: angličtina
Zdroj: Cell and tissue banking [Cell Tissue Bank] 2021 Sep; Vol. 22 (3), pp. 409-417. Date of Electronic Publication: 2021 Jan 01.
DOI: 10.1007/s10561-020-09887-4
Abstrakt: Mesenchymal stem cells, being characterized by high self-renewal capacity and multi-lineage differentiation potential, are widely used in regenerative medicine especially for repair of bone defects in patients with poor bone regenerative capacity. In this study, we aimed to compare the osteogenic potential of human maxillary schneiderian sinus membrane (hMSSM)-derived stem cells versus permanent teeth dental pulp stem cells (DPSCs). Both cells types were cultivated in osteogenic and non-osteogenic inductive media. Alkaline phosphatase (ALP) activity assay and quantitative real-time PCR analysis were carried out to assess osteogenic differentiation. We showed that ALP activity and osteoblastic markers transcription levels were more striking in hMSSM-derived stem cells than DPSCs. Our results highlight hMSSM-derived stem cells as a recommended stem cell type for usage during bone tissue regenerative therapy.
(© 2021. The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: