HASTY modulates miRNA biogenesis by linking pri-miRNA transcription and processing.

Autor: Cambiagno DA; Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina., Giudicatti AJ; Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina., Arce AL; Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina., Gagliardi D; Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina., Li L; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany., Yuan W; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany., Lundberg DS; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany., Weigel D; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany., Manavella PA; Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina. Electronic address: pablomanavella@ial.santafe-conicet.gov.ar.
Jazyk: angličtina
Zdroj: Molecular plant [Mol Plant] 2021 Mar 01; Vol. 14 (3), pp. 426-439. Date of Electronic Publication: 2020 Dec 30.
DOI: 10.1016/j.molp.2020.12.019
Abstrakt: Post-transcriptional gene silencing mediated by microRNAs (miRNAs) modulates numerous developmental and stress response pathways. For the last two decades, HASTY (HST), the ortholog of human EXPORTIN 5, was considered to be a candidate protein that exports plant miRNAs from the nucleus to the cytoplasm. Here, we report that HST functions in the miRNA pathway independent of its cargo-exporting activity in Arabidopsis. We found that Arabidopsis mutants with impaired HST shuttling exhibit normal subcellular distribution of miRNAs. Interestingly, protein-protein interaction and microscopy assays showed that HST directly interacts with the microprocessor core component DCL1 through its N-terminal domain. Moreover, mass spectrometry analysis revealed that HST also interacts independently of its N-terminal domain with the mediator complex subunit MED37. Further experiments revealed that HST could act as a scaffold to facilitate the recruitment of DCL1 to genomic MIRNA loci by stabilizing the DCL1-MED37 complex, which in turn promotes the transcription and proper processing of primary miRNA transcripts (pri-miRNAs). Taken together, these results suggest that HST is likely associated with the formation of the miRNA biogenesis complex at MIRNA genes, promoting the transcription and processing of pri-miRNAs rather than the direct export of processed miRNAs from the nucleus.
(Copyright © 2021 The Author. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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