The protealysin operon encodes emfourin, a prototype of a novel family of protein metalloprotease inhibitors.

Autor: Chukhontseva KN; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Berdyshev IM; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Safina DR; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Karaseva MA; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Bozin TN; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia; National Research Centre 'Kurchatov Institute', Moscow, Russia., Salnikov VV; Kazan Federal University, Kazan, Russia; Kazan Institute of Biochemistry and Biophysics, Federal Research Center 'Kazan Scientific Center of the Russian Academy of Sciences', Kazan, Russia., Konarev PV; Shubnikov Institute of Crystallography of the Federal Scientific Research Centre 'Crystallography and Photonics', Russian Academy of Sciences, Moscow, Russia., Volkov VV; Shubnikov Institute of Crystallography of the Federal Scientific Research Centre 'Crystallography and Photonics', Russian Academy of Sciences, Moscow, Russia., Grishin AV; Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia., Kozlovskiy VI; Chernogolovka Branch of the Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Chernogolovka, Russia., Kostrov SV; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia., Demidyuk IV; Institute of Molecular Genetics of National Research Centre 'Kurchatov Institute', Moscow, Russia. Electronic address: duk@img.ras.ru.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2021 Feb 01; Vol. 169, pp. 583-596. Date of Electronic Publication: 2020 Dec 29.
DOI: 10.1016/j.ijbiomac.2020.12.170
Abstrakt: Protealysin is a Serratia proteamaculans metalloproteinase of the M4 peptidase family and the prototype of a large group of protealysin-like proteases (PLPs). PLPs are likely involved in bacterial interaction with plants and animals as well as in bacterial pathogenesis. We demonstrated that the PLP genes in bacteria colocalize with the genes of putative conserved proteins. In S. proteamaculans, these two genes form a bicistronic operon. The putative S. proteamaculans protein that we called emfourin (M4in) was expressed in Escherichia coli and characterized. M4in forms a complex with protealysin with a 1:1 stoichiometry and is a potent slow-binding competitive inhibitor of protealysin (K i = 52 ± 14 pM); besides, M4in is not secreted from S. proteamaculans constitutively. A comparison of amino acid sequences of M4in and its homologs with those of known inhibitors suggests that M4in is the prototype of a new family of protein inhibitors of proteases.
Competing Interests: Declaration of competing interest All authors declare no competing interests.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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