Comparative karyotype study of three Cyprinids (Cyprinidae, Cyprininae) in Thailand by classical cytogenetic and FISH techniques.

Autor: Phimphan S; Biology program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000, Thailand Phetchabun Rajabhat University Phetchabun Thailand., Chaiyasan P; Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand Khon Kaen University Khon Kaen Thailand., Suwannapoom C; Department of Fishery, School of Agriculture and Natural Resources, University of Phayao, Muang, Phayao 56000, Thailand University of Phayao Phayao Thailand., Reungsing M; Department of Biotechnology, Faculty of Science and Technology, Rajamangala University of Technology Tawan-ok, Sri Racha, Chon Buri 20110, Thailand Rajamangala University Chon Buri Thailand., Juntaree S; Faculty of Interdisciplinary Studies, Nong Khai Campus, Khon Kaen, University, Muang, Nong Khai 43000, Thailand Khon Kaen University Nong Khai Thailand., Tanomtong A; Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand Khon Kaen University Khon Kaen Thailand., Supiwong W; Faculty of Interdisciplinary Studies, Nong Khai Campus, Khon Kaen, University, Muang, Nong Khai 43000, Thailand Khon Kaen University Nong Khai Thailand.
Jazyk: angličtina
Zdroj: Comparative cytogenetics [Comp Cytogenet] 2020 Dec 22; Vol. 14 (4), pp. 597-612. Date of Electronic Publication: 2020 Dec 22 (Print Publication: 2020).
DOI: 10.3897/CompCytogen.v14i4.54428
Abstrakt: Three species of ornamental fishes in the subfamily Cyprininae (family Cyprinidae) namely, Epalzeorhynchos frenatum (Fowler, 1934), Puntigrus partipentazona (Fowler, 1934), Scaphognathops bandanensis Boonyaratpalin et Srirungroj, 1971 were studied by classical cytogenetic and fluorescent in situ hybridization (FISH) techniques. Chromosomes were directly prepared from kidney tissues and stained by using conventional and Ag-NOR banding techniques. Microsatellite d(CA) 15 and d(CGG) 10 probes were hybridized to the chromosomes of three cyprinids. The results show that the three cyprinid species share the same diploid number as 2n=50 but there are differences in the fundamental number (NF) and karyotypes i.e. E. frenatum : NF = 78, 18m+10sm+10st+12a; P. partipentazona : NF = 80, 6m+24sm+14st+6a; S. bandanensis : NF = 66, 4m+12sm+34a. NOR positive masks were observed at the regions adjacent to the telomere of the short arm of the chromosome pairs 10 (submetacentric) and 1 (metacentric) in E. frenatum and P. partipentazona , respectively whereas those were revealed at telomeric regions of the long arm of the chromosome pair 9 (acrocentric) in S. bandanensis . The mapping of d(CA) 15 and d(CGG) 10 microsatellites shown that hybridization signals are abundantly distributed in telomeric regions of several pairs except d(CA) 15 repeats in S. bandanensis , which are distributed throughout all chromosomes and d(CGG) 10 repeats in P. partipentazona display the high accumulation only in the first chromosome pair.
(Sumalee Phimphan, Patcharaporn Chaiyasan, Chatmongkon Suwannapoom, Montri Reungsing, Sippakorn Juntaree, Alongklod Tanomtong, Weerayuth Supiwong.)
Databáze: MEDLINE
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