Rumen bacterial diversity in relation to nitrogen retention in beef cattle.

Autor: Gomes Carvalho Alves KL; Department of Animal Science, School of Agrarian and Veterinarian Sciences, São Paulo State University - UNESP, Jaboticabal, SP, Brazil. Electronic address: kenialgcalves@hotmail.com., Granja-Salcedo YT; Department of Animal Science, School of Agrarian and Veterinarian Sciences, São Paulo State University - UNESP, Jaboticabal, SP, Brazil; Corporación Colombiana de Investigación Agropecuaria, AGROSAVIA, CI EI Nus, San Roque, Antioquía, Colombia., Messana JD; Department of Animal Science, School of Agrarian and Veterinarian Sciences, São Paulo State University - UNESP, Jaboticabal, SP, Brazil., Carneiro de Souza V; Department of Animal Science, School of Agrarian and Veterinarian Sciences, São Paulo State University - UNESP, Jaboticabal, SP, Brazil; Department of Dairy Science, Virginia Tech, Blacksburg, 24061, USA., Generoso Ganga MJ; Department of Animal Science, School of Agrarian and Veterinarian Sciences, São Paulo State University - UNESP, Jaboticabal, SP, Brazil., Detogni Colovate PH; Department of Animal Science, School of Agrarian and Veterinarian Sciences, São Paulo State University - UNESP, Jaboticabal, SP, Brazil., Kishi LT; National Laboratory of Scientific Computing, Petrópolis, RJ, Brazil; Department of Technology, School of Agrarian and Veterinarian Sciences, São Paulo State University - UNESP, Jaboticabal, SP, Brazil., Berchielli TT; Department of Animal Science, School of Agrarian and Veterinarian Sciences, São Paulo State University - UNESP, Jaboticabal, SP, Brazil; INCT/CA-UFV-Department of Animal Science, Viçosa, MG, Brazil.
Jazyk: angličtina
Zdroj: Anaerobe [Anaerobe] 2021 Feb; Vol. 67, pp. 102316. Date of Electronic Publication: 2020 Dec 29.
DOI: 10.1016/j.anaerobe.2020.102316
Abstrakt: This study aimed to characterize the rumen bacterial diversity of beef steers differing in the efficiency of nitrogen retention (ENR). Eight castrated steers and fitted with ruminal silicone - and duodenal T-type cannulas were used in a cross-over design with three consecutive periods and three diets. During each experimental period, nitrogen balance was measured, and based on the efficiency of N utilization data, steers were split into three ENR groups: high (HNR, 56.6% ± 3.3%, n = 10), medium (MNR, 45.8% ± 2.2%, n = 6), and low (LNR, 37.7% ± 1.9%, n = 8) using the NbClust package version 2.0.4 in R. Prevotellaceae, Lactobacillaceae, Leuconostocaceae, Clostridiales_Incertae_Sedis_XIII, Lachnospiraceae, and Peptostreptococcaceae were more abundant in LNR (P < 0.05) compared to HNR or MNR. Negative correlations were found between N retention and Mogibacterium, Anaerofustis, Butyrivibrio, Coprococcus, Hespellia, Lactonifactor and Lachnospiraceae (r ≤ -0.61; P ≤ 0.05). Prevotella, Hespellia, Lactonifactor, Lachnospiraceae_other, and Anaerobiospirillum were positively correlated between urinary N excretion (r > 0.55; P < 0.01), and negative correlations were found with Elusimicrobia, Victivallis and Treponema (r < -0.41; P < 0.05). The adjustment of the rumen bacterial community differed significantly between the N use retention groups. The high N retention in beef cattle was associated with less abundant bacteria in the rumen; however, N fixation capacity and uncharacterized rumen microorganisms need to be elucidated in future studies. In contrast, lower N utilization was associated with high abundance of bacteria that promote greater urinary N excretion through ruminal protein degradation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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