Development of fibronectin-loaded nanofiber scaffolds for guided pulp tissue regeneration.

Autor: Leite ML; Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, Universidade Estadual Paulista, Araraquara, Brazil., Soares DG; Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, Sao Paulo University, Bauru, Brazil., Anovazzi G; Departament of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, São Paulo State University, Araraquara, Brazil., Mendes Soares IP; Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, Universidade Estadual Paulista, Araraquara, Brazil., Hebling J; Departament of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, São Paulo State University, Araraquara, Brazil., de Souza Costa CA; Department of Physiology and Pathology, Araraquara School of Dentistry, São Paulo State University, Araraquara, Brazil.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2021 Sep; Vol. 109 (9), pp. 1244-1258. Date of Electronic Publication: 2020 Dec 31.
DOI: 10.1002/jbm.b.34785
Abstrakt: Fibronectin (FN)-loaded nanofiber scaffolds were developed and assessed concerning their bioactive potential on human apical papilla cells (hAPCs). First, random (NR) and aligned (NA) nanofiber scaffolds of polycaprolactone (PCL) were obtained by electrospinning technique and their biological properties were evaluated. The best formulations of NR and NA were loaded with 0, 5, or 10 μg/ml of FN and their bioactivity was assessed. Finally, FN-loaded NR and NA tubular scaffolds were prepared and their chemotactic potential was analyzed using an in vitro model to mimic the pulp regeneration of teeth with incomplete root formation. All scaffolds tested were cytocompatible. However, NR and NA based on 10% PCL promoted the highest hAPCs proliferation, adhesion and spreading. Polygonal and elongated cells were observed on NR and NA, respectively. The higher the concentration of FN added to the scaffolds, greater cell migration, viability, proliferation, adhesion and spreading, as well as collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1). In addition, tubular scaffolds with NA loaded with FN (10 μg/ml) showed the highest chemotactic potential on hAPCs. It was concluded that FN-loaded NA scaffolds may be an interesting biomaterial to promote hAPCs-mediated pulp regeneration of endodontically compromised teeth with incomplete root formation.
(© 2020 Wiley Periodicals LLC.)
Databáze: MEDLINE
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