Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasets.

Autor:	Mou MA; Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Bangladesh., Keya NA; Department of Microbiology, Noakhali Science and Technology University, Bangladesh., Islam M; Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail, 1902, Bangladesh., Hossain MJ; Maternal and Child Health Division (MCHD), Icddrb, Bangladesh., Al Habib MS; Department of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail, 1902, Bangladesh., Alam R; Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh.; Laboraty of Computational Biology, Biological Solution Cantre (BiolSol Centre), Dhaka, Bangladesh., Rana S; Department of Pharmacy, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh., Samad A; Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh.; Laboraty of Computational Biology, Biological Solution Cantre (BiolSol Centre), Dhaka, Bangladesh., Ahammad F; Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh.; Laboraty of Computational Biology, Biological Solution Cantre (BiolSol Centre), Dhaka, Bangladesh.; Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, 21589, Saudi Arabia.
Jazyk:	angličtina
Zdroj:	Biochemistry and biophysics reports [Biochem Biophys Rep] 2020 Dec 02; Vol. 24, pp. 100867. Date of Electronic Publication: 2020 Dec 02 (Print Publication: 2020).
DOI:	10.1016/j.bbrep.2020.100867
Abstrakt:	Breast cancer ranked second among most frequent cancer in the world playing a significant role in mortality rate. Having prior knowledge on differentially expressed genes in breast cell carcinoma elucidated important indications to understand the molecular mechanism underneath breast carcinogenesis. In this study we have investigated the distinguished CSN1S1 expression in human breast cancer. We have analyzed CSN1S1 mRNA expression between cancer and normal tissues using TCGA datasets. Moreover, analysis including promoter methylation, mutations, prognosis, co-expression, gene ontology, and pathways of CSN1S1 were performed by the TCGA Wanderer, UCSC Xena, cBioPortal, PrognoScan, UALCAN, and Enricher server. We have observed low mRNA expression and high promoter methylation of CSN1S1 in cancer tissues compared to normal tissues. Furthermore, we have also identified low mRNA expression in clinicopathological patients, as well as 9 deleterious mutations with highly co-expressed protein MRC1, and significantly related signaling pathways. We have found a positive correlation between the lower expression of CSN1S1 and patients surviving with breast cancer. Here we have concluded that CSN1S1 acts as a biomarker for the surveillance and prognosis of breast cancer, and also works as a novel therapeutic target at the molecular and pathway levels. Competing Interests: Authors declare no conflict of interest. (© 2020 The Authors.)
Databáze:	MEDLINE
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