Investigating the anti-inflammatory effects of high molecular weight secretions from Limosilactobacillus reuteri PTCC 1655 on LPS-stimulated PMA-differentiated THP-1 cells.

Autor: Johari B; Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.; Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran., Maghsood F; Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.; Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran., Madanchi H; Department of Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.; Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran., Moradi M; Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran., Kadivar M; Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2021 Aug; Vol. 131 (2), pp. 938-948. Date of Electronic Publication: 2021 Jan 11.
DOI: 10.1111/jam.14984
Abstrakt: Aims: This study was done to investigate the anti-inflammatory effects of high molecular weight secretions from Limosilactobacillus reuteri PTCC 1655 probiotic bacteria on lipopolysaccharide (LPS)-stimulated phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 cells.
Methods and Results: After culturing the bacterium, the crude cell-free supernatant was fractionated on the basis of molecular weights using ultrafiltration. Also, a heat-killed and sonicated fraction was obtained from the biomass of the bacterial culture. All fractions were used to measure their anti-inflammatory effects on PMA-differentiated THP-1 cells following LPS stimulation by quantifying various cellular markers of inflammation. The results demonstrated that various L. reuteri PTCC 1655-derived fractions, especially the >100 kDa supernatant fraction decreased some of the inflammatory cytokines and mediators, including tumour necrosis factor-α, interleukin-1, nitric oxide, cyclooxygenase-2, matrix metalloproteinase-9 and interleukin-6, which are critical for the pathogenesis of some inflammatory diseases.
Conclusion: It is concluded that the L. reuteri PTCC 1655-derived high molecular weight fractions significantly reduce inflammation and therefore could be appropriate candidates for future medical studies.
Significance and Impact of the Study: Providing new insights about the significance of L. reuteri PTCC 1655-derived extracts and their potential to modulate inflammation.
(© 2020 The Society for Applied Microbiology.)
Databáze: MEDLINE
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