Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts.

Autor:	Reusch S; IRI Life Sciences, Humboldt-Universität zu Berlin, 10115 Berlin, Germany.; Marine Biological Laboratory, Woods Hole, MA 02543, USA., Biswas A; IRI Life Sciences, Humboldt-Universität zu Berlin, 10115 Berlin, Germany.; Max-Planck-Institute for the Physics of Light, 91058 Erlangen, Germany.; Marine Biological Laboratory, Woods Hole, MA 02543, USA., Hirst WG; IRI Life Sciences, Humboldt-Universität zu Berlin, 10115 Berlin, Germany.; Research School of Biology, The Australian National University, Canberra, ACT 2600, Australia.; Marine Biological Laboratory, Woods Hole, MA 02543, USA., Reber S; IRI Life Sciences, Humboldt-Universität zu Berlin, 10115 Berlin, Germany.; University of Applied Sciences Berlin, 13353 Berlin, Germany.; Marine Biological Laboratory, Woods Hole, MA 02543, USA.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2020 Oct 31; Vol. 1 (3), pp. 100151. Date of Electronic Publication: 2020 Oct 31 (Print Publication: 2020).
DOI:	10.1016/j.xpro.2020.100151
Abstrakt:	Cytoplasmic extracts from unfertilized Xenopus eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these in vitro studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologically relevant Xenopus tubulins in milligram yield, which are a complex mixture of isoforms with various post-translational modifications. The protocol is applicable to any cell or tissue of interest. For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020). Competing Interests: The authors declare no competing interests. (© 2020 The Authors.)
Databáze:	MEDLINE
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