Isolation, Culture, and Functional Analysis of Murine Thermogenic Adipocytes.

Autor:	Oeckl J; Chair for Molecular Nutritional Medicine, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; ZIEL-Institute for Food & Health, Technical University of Munich, Freising, Germany., Bast-Habersbrunner A; Chair for Molecular Nutritional Medicine, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; ZIEL-Institute for Food & Health, Technical University of Munich, Freising, Germany., Fromme T; Chair for Molecular Nutritional Medicine, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; ZIEL-Institute for Food & Health, Technical University of Munich, Freising, Germany., Klingenspor M; Chair for Molecular Nutritional Medicine, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; ZIEL-Institute for Food & Health, Technical University of Munich, Freising, Germany., Li Y; Chair for Molecular Nutritional Medicine, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Gregor-Mendel-Str. 2, 85354 Freising, Germany.; ZIEL-Institute for Food & Health, Technical University of Munich, Freising, Germany.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2020 Sep 22; Vol. 1 (3), pp. 100118. Date of Electronic Publication: 2020 Sep 22 (Print Publication: 2020).
DOI:	10.1016/j.xpro.2020.100118
Abstrakt:	Studying brown and brite adipose tissue requires precise and reliable quantification of cellular thermogenesis. This protocol describes the isolation of primary murine pre-adipocytes, differentiation into thermogenic brown and brite adipocytes, and subsequent oxygen consumption analysis. Commonly applied procedures only measure basal and maximal proton leak-linked oxygen consumption but not explicitly uncoupling protein 1 (UCP1)-dependent respiration. Meaningful oxygen consumption analyses require (1) the activation of UCP1, (2) control over intracellular free-fatty-acid levels, and (3) inhibition of ATP-consuming futile cycles. For complete details on the use and execution of this protocol, please refer to Li et al. (2014, 2017, 2018) and Schweizer et al. (2018). Competing Interests: The authors declare no competing interests. (© 2020 The Author(s).)
Databáze:	MEDLINE
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