| Autor: |
Kisrieva YS; Institute of Biomedical Chemistry, Moscow, Russia., Samenkova NF; Institute of Biomedical Chemistry, Moscow, Russia., Larina OB; Institute of Biomedical Chemistry, Moscow, Russia., Zgoda VG; Institute of Biomedical Chemistry, Moscow, Russia., Karuzina II; Institute of Biomedical Chemistry, Moscow, Russia., Rusanov AL; LLC 'Perspective', Novosibirsk, Russia., Luzgina NG; Institute of Biomedical Chemistry, Moscow, Russia., Petushkova NA; Institute of Biomedical Chemistry, Moscow, Russia. |
| Abstrakt: |
Using electrospray ionization tandem mass spectrometry, a comparative analysis of the HaCaT keratinocyte proteins encoded by the 18th chromosome was performed before and after exposure to sodium dodecyl sulfate (25 mg/ml) and to Triton X-100 (12.5 mg/ml) in a subtoxic dose for 48 hours. Proteins were identified using the SearchGUI platform (X!Tandem and MS-GF+ search engines). In total, 1284 proteins were found in immortalized human HaCaT keratinocytes and about 75% of them were identified by two or more peptides. Were identified, that 26 proteins were encoded by genes of chromosome 18. Among these proteins, 17 were common for control cells and HaCaT cells treated with SDS. Proteins MARE2 and CTIF were identified only in control keratinocytes. Seven identified proteins encoded by genes of chromosome 18 were found only in detergent-treated keratinocytes: LMAN1, NDUV2, SPB3, VPS4B, KDSR, ROCK1 and RHG28. |
