Comprehensive phenotyping revealed transient startle response reduction and histopathological gadolinium localization to perineuronal nets after gadodiamide administration in rats.

Autor: Habermeyer J; Department of Experimental Therapy, University Hospital Erlangen and Preclinical Experimental Animal Center (PETZ), Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Universitätsklinikum Erlangen (UKEr), Palmsanlage 5, 91054, Erlangen, Germany., Boyken J; Bayer AG, MR & CT Contrast Media Research, Muellerstrasse 178, 13353, Berlin, Germany., Harrer J; Department of Experimental Therapy, University Hospital Erlangen and Preclinical Experimental Animal Center (PETZ), Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Universitätsklinikum Erlangen (UKEr), Palmsanlage 5, 91054, Erlangen, Germany., Canneva F; Department of Experimental Therapy, University Hospital Erlangen and Preclinical Experimental Animal Center (PETZ), Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Universitätsklinikum Erlangen (UKEr), Palmsanlage 5, 91054, Erlangen, Germany., Ratz V; Department of Experimental Therapy, University Hospital Erlangen and Preclinical Experimental Animal Center (PETZ), Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Universitätsklinikum Erlangen (UKEr), Palmsanlage 5, 91054, Erlangen, Germany., Moceri S; Department of Experimental Therapy, University Hospital Erlangen and Preclinical Experimental Animal Center (PETZ), Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Universitätsklinikum Erlangen (UKEr), Palmsanlage 5, 91054, Erlangen, Germany., Admard J; Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany., Casadei N; Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.; DFG NGS Competence Center Tuebingen, 72076, Tuebingen, Germany., Jost G; Bayer AG, MR & CT Contrast Media Research, Muellerstrasse 178, 13353, Berlin, Germany., Bäuerle T; Department of. Radiology, University Hospital Erlangen and Preclinical Imaging Center Erlangen (PIPE), Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Universitätsklinikum Erlangen (UKEr), Erlangen, Germany., Frenzel T; Bayer AG, MR & CT Contrast Media Research, Muellerstrasse 178, 13353, Berlin, Germany., Schmitz C; Department of Neuroanatomy, Ludwig-Maximilians-University, Munich, Germany., Schütz G; Bayer AG, MR & CT Contrast Media Research, Muellerstrasse 178, 13353, Berlin, Germany., Pietsch H; Bayer AG, MR & CT Contrast Media Research, Muellerstrasse 178, 13353, Berlin, Germany., von Hörsten S; Department of Experimental Therapy, University Hospital Erlangen and Preclinical Experimental Animal Center (PETZ), Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Universitätsklinikum Erlangen (UKEr), Palmsanlage 5, 91054, Erlangen, Germany. stephan.v.hoersten@fau.de.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Dec 28; Vol. 10 (1), pp. 22385. Date of Electronic Publication: 2020 Dec 28.
DOI: 10.1038/s41598-020-79374-z
Abstrakt: Gadolinium based contrast agents (GBCAs) are widely used in clinical MRI since the mid-1980s. Recently, concerns have been raised that trace amounts of Gadolinium (Gd), detected in brains even long time after GBCA application, may cause yet unrecognized clinical consequences. We therefore assessed the behavioral phenotype, neuro-histopathology, and Gd localization after repeated administration of linear (gadodiamide) or macrocyclic (gadobutrol) GBCA in rats. While most behavioral tests revealed no difference between treatment groups, we observed a transient and reversible decrease of the startle reflex after gadodiamide application. Residual Gd in the lateral cerebellar nucleus was neither associated with a general gene expression pathway deregulation nor with neuronal cell loss, but in gadodiamide-treated rats Gd was associated with the perineuronal net protein aggrecan and segregated to high molecular weight fractions. Our behavioral finding together with Gd distribution and speciation support a substance class difference for Gd presence in the brain after GBCA application.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje