Ameliorative effect of melatonin on apoptosis, DNA fragmentation, membrane integrity and lipid peroxidation of spermatozoa in the idiopathic asthenoteratospermic men: In vitro.

Autor: Malmir M; Department of Midwifery,Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran., Naderi Noreini S; Rastak Infertility Center, Arak, Iran., Ghafarizadeh A; Academic Center for Education, Culture and Research, Arak, Iran., Faraji T; Department of Midwifery,Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran., Asali Z; Department of Midwifery,Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran.
Jazyk: angličtina
Zdroj: Andrologia [Andrologia] 2021 Mar; Vol. 53 (2), pp. e13944. Date of Electronic Publication: 2020 Dec 27.
DOI: 10.1111/and.13944
Abstrakt: Fertility loss, recurrent spontaneous abortion and poor outcome in assisted reproductive techniques (ART) have been associated with DNA fragmentation. This work was achieved to evaluate the protective role of melatonin versus apoptosis, DNA fragmentation, membrane integrity and lipid peroxidation of spermatozoa from men with asthenoteratozoospermia (ATS). Some researchers maintain that melatonin can serve as a remedy for apoptosis induction, and it has an impressive effect on boosting the quality and quantity of spermatozoa. For this purpose, semen samples were collected from 50 ATS men and they were divided into control and melatonin (6 mM) groups at 2, 4, 6 and 24 hr. Concentrating on the reasons for apoptosis is an arduous process, but in the present study for this evaluation mitochondrial membrane potential (MMP), DNA fragmentation by TUNEL and sperm chromatin dispersion (SCD) methods and lipid peroxidation were carried out. Also, sperm viability was performed. In the control group, MDA, TUNEL-positive and SCD were significantly increased but viability and MMP of spermatozoa were significantly decreased. Moreover, in the melatonin group, TUNEL-positive, SCD and MDA levels were significantly decreased and viability and MMP significantly increased, compared to the control group. In outcome, melatonin prescription paves the way for apoptosis down-regulating in the ATS men.
(© 2020 Wiley-VCH GmbH.)
Databáze: MEDLINE
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