High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow.

Autor:	Panvini FM; Institute of Life Sciences, Sant'Anna School of Advanced Studies, Pisa, Italy.; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy., Pacini S; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy., Montali M; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy., Barachini S; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy., Mazzoni S; Department of Translational Research and New Technology in Medicine, University of Pisa, Pisa, Italy., Morganti R; Statistical Support to Clinical Trials Department, Azienda Ospedaliero Universitaria Pisana, Pisa, Italy., Ciancia EM; Department of Pathology, Azienda Ospedsaliero Universitaria Pisana, Pisa, Italy., Carnicelli V; Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, Italy., Petrini M; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in cell and developmental biology [Front Cell Dev Biol] 2020 Dec 08; Vol. 8, pp. 596452. Date of Electronic Publication: 2020 Dec 08 (Print Publication: 2020).
DOI:	10.3389/fcell.2020.596452
Abstrakt:	Hematopoiesis is hosted, supported and regulated by a special bone marrow (BM) microenvironment known as "niche." BM niches have been classified based on micro-anatomic distance from the bone surface into "endosteal" and "central" niches. Whilst different blood vessels have been found in both BM niches in mice, our knowledge of the human BM architecture is much more limited. Here, we have used a combination of markers including NESTIN, CD146, and αSMA labeling different blood vessels in benign human BM. Applying immunohistochemical/immunofluorescence techniques on BM trephines and performing image analysis on almost 300 microphotographs, we detected high NESTIN expression in BM endothelial cells (BMECs) of small arteries (A) and endosteal arterioles (EA), and also in very small vessels we named NESTIN + capillary-like tubes (NCLTs), not surrounded by sub-endothelial perivascular cells that occasionally reported low levels of NESTIN expression. Statistically, NCLTs were detected within 40 μm from bone trabecula, frequently found in direct contact to the bone line and spatially correlated with hematopoietic stem/progenitor cells. Our results support the expression of NESTIN in human BMECs of EA and A in accordance with the updated classification of murine BM micro-vessels. NCLTs for their peculiar characteristics and micro-anatomical localization have been here proposed as transitional vessels possibly involved in regulating human hematopoiesis. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2020 Panvini, Pacini, Montali, Barachini, Mazzoni, Morganti, Ciancia, Carnicelli and Petrini.)
Databáze:	MEDLINE
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