Analysis of the structure, loading and activity of six antimicrobial peptides encapsulated in cubic phase lipid nanoparticles.

Autor: Meikle TG; RMIT University, School of Science, College of Science Engineering and Health, 124 La Trobe Street, Melbourne, Victoria 3000, Australia., Dharmadana D; RMIT University, School of Science, College of Science Engineering and Health, 124 La Trobe Street, Melbourne, Victoria 3000, Australia., Hoffmann SV; ISA, Department of Physics and Astronomy, Aarhus University, Ny Munkegade 120, DK-8000 Aarhus C, Denmark., Jones NC; ISA, Department of Physics and Astronomy, Aarhus University, Ny Munkegade 120, DK-8000 Aarhus C, Denmark., Drummond CJ; RMIT University, School of Science, College of Science Engineering and Health, 124 La Trobe Street, Melbourne, Victoria 3000, Australia., Conn CE; RMIT University, School of Science, College of Science Engineering and Health, 124 La Trobe Street, Melbourne, Victoria 3000, Australia. Electronic address: charlotte.conn@rmit.edu.au.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2021 Apr; Vol. 587, pp. 90-100. Date of Electronic Publication: 2020 Dec 02.
DOI: 10.1016/j.jcis.2020.11.124
Abstrakt: The growing global threat of antimicrobial resistance, combined with the slowed development of novel antibiotics, has resulted in a critical need for new antimicrobial therapies. Naturally occurring antimicrobial peptides (AMPs) can act as highly potent, broad-spectrum antibiotics which may be less likely to engender resistance in target organisms. However, their susceptibility to proteolysis and lack of specificity necessitates the use of a drug delivery vehicle to both protect the AMP from chemical degradation and provide a platform for further functionalization, enabling the development of targeted delivery and release systems. In this study, we have used lipid-based inverse bicontinuous cubic phase nanoparticles (cubosomes) as delivery vehicles for six different antimicrobial peptides. The phase stability, morphology, and peptide loading efficiency of the nanoparticles were characterized and rationalized according to lipid composition, buffer conditions, as well as peptide charge and hydrophobicity. The AMP loading efficiency within cubosomes was increased significantly through simple manipulation of electrostatic charge. Minimum inhibitory concentration (MIC) values were determined for formulations with high loading efficiency against Staphylococcus aureus, Bacilus cereus, Escherichia coli, and Pseudomonas aeruginosa. Encapsulation within a lipid nanocarrier was shown to increase antimicrobial activity for some formulations. We anticipate that the further development of these peptide loaded cubosomes will enable the design of potent and targeted antibiotic therapies.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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