Identification and quantification of neuronal ensembles in optical imaging experiments.

Autor: Wenzel M; Department of Epileptology, University Hospital Bonn, 53127, Bonn, Germany. Electronic address: michaelwenzel2946@gmail.com., Hamm JP; Neuroscience Institute, Georgia State University, Atlanta, GA, 30303, USA. Electronic address: jhamm1@gsu.edu.
Jazyk: angličtina
Zdroj: Journal of neuroscience methods [J Neurosci Methods] 2021 Mar 01; Vol. 351, pp. 109046. Date of Electronic Publication: 2020 Dec 24.
DOI: 10.1016/j.jneumeth.2020.109046
Abstrakt: Recent technical advances in molecular biology and optical imaging have made it possible to record from up to thousands of densely packed neurons in superficial and deep brain regions in vivo, with cellular subtype specificity and high spatiotemporal fidelity. Such optical neurotechnologies are enabling increasingly fine-scaled studies of neuronal circuits and reliably co-active groups of neurons, so-called ensembles. Neuronal ensembles are thought to constitute the basic functional building blocks of brain systems, potentially exhibiting collective computational properties. While the technical framework of in vivo optical imaging and quantification of neuronal activity follows certain widely held standards, analytical methods for study of neuronal co-activity and ensembles lack consensus and are highly varied across the field. Here we provide a comprehensive step-by-step overview of theoretical, experimental, and analytical considerations for the identification and quantification of neuronal ensemble dynamics in high-resolution in vivo optical imaging studies.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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