Isolation and identification of quorum sensing antagonist from Cinnamomum verum leaves against Pseudomonas aeruginosa.

Autor: Alva PP; NITTE (Deemed to be University), Nitte University Centre for Science Education and Research, Paneer campus, Deralakatte, Mangaluru 575018, Karnataka, India., Suresh S; NITTE (Deemed to be University), Nitte University Centre for Science Education and Research, Paneer campus, Deralakatte, Mangaluru 575018, Karnataka, India., Nanjappa DP; NITTE (Deemed to be University), Nitte University Centre for Science Education and Research, Paneer campus, Deralakatte, Mangaluru 575018, Karnataka, India., James JP; NITTE (Deemed to be University), Nitte Gulabi Shetty Memorial Institute of Pharmaceutical Sciences, Paneer campus, Deralakatte, Mangaluru 575018, Karnataka, India., Kaverikana R; NITTE (Deemed to be University), Nitte Gulabi Shetty Memorial Institute of Pharmaceutical Sciences, Paneer campus, Deralakatte, Mangaluru 575018, Karnataka, India., Chakraborty A; NITTE (Deemed to be University), Nitte University Centre for Science Education and Research, Paneer campus, Deralakatte, Mangaluru 575018, Karnataka, India., Sarojini BK; Department of Industrial Chemistry, Mangalore University, Mangalagangotri 574199, Karnataka, India., Premanath R; NITTE (Deemed to be University), Nitte University Centre for Science Education and Research, Paneer campus, Deralakatte, Mangaluru 575018, Karnataka, India. Electronic address: ramya@nitte.edu.in.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2021 Feb 15; Vol. 267, pp. 118878. Date of Electronic Publication: 2020 Dec 27.
DOI: 10.1016/j.lfs.2020.118878
Abstrakt: Purpose: The study aimed at isolating and identifying potential anti-quorum sensing (QS) compounds from Cinnamomum verum leaves against Pseudomonas aeruginosa.
Methodology: Isolation of anti-QS compounds from C. verum leaf ethanol extract was carried out by column chromatography. The bioactive fraction was analysed by UV, IR, and GCMS spectroscopy. Various virulence assays were performed to assess the QS quenching ability of the purified compounds. In vivo toxicity of the purified compounds was examined in zebrafish model. The expression of the virulence genes was evaluated by qPCR analysis and in silico assessment was accomplished to check the binding ability of the compounds with the autoinducer molecule.
Key Findings: The QS inhibitors isolated and identified showed a remarkable ability in reducing the production of elastase, pyocyanin, swarming motility and biofilm formation in P. aeruginosa. In the presence of the characterized compounds, the expression of virulence genes of P. aeruginosa was significantly reduced. Toxicity studies in zebrafish model indicated no effects on development and organogenesis at a concentration below 100 mg/l. Further, in silico analysis demonstrated the binding efficiency of the anti-QS compounds to AHL molecules, thus proving the QS quenching ability of the isolated compounds.
Significance: To the best of our knowledge this is the first report of isolation of anti-QS compounds from C. verum leaves against P. aeruginosa. The identified compounds qualify as potential QS antagonists. Further studies on these compounds can pave way for an effective and attractive anti-pathogenic therapy, to overcome the emergence of antibiotic resistance in bacteria.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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