Structural, biosynthetic and serological cross-reactive elucidation of capsular polysaccharides from Streptococcus pneumoniae serogroup 28.

Autor: Li C; Department of Chemistry, Technical University of Denmark, Kgs. Lyngby, Denmark., Duda KA; Junior Research Group of Allergobiochemistry, Research Center Borstel, Leibniz Lungenzentrum, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Borstel, Germany., Elverdal PL; SSI Diagnostica A/S, Hilleroed, Denmark., Skovsted IC; SSI Diagnostica A/S, Hilleroed, Denmark., Kjeldsen C; Department of Chemistry, Technical University of Denmark, Kgs. Lyngby, Denmark., Teze D; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby, Denmark., Duus JØ; Department of Chemistry, Technical University of Denmark, Kgs. Lyngby, Denmark. Electronic address: jduus@kemi.dtu.dk.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2021 Feb 15; Vol. 254, pp. 117323. Date of Electronic Publication: 2020 Nov 04.
DOI: 10.1016/j.carbpol.2020.117323
Abstrakt: Capsular polysaccharides (CPS) are the key virulent factors in the pathogenesis of Streptococcus pneumoniae. The previously unknown CPS structures of the pneumococcal serotype 28F and 28A were thoroughly characterized by NMR spectroscopy, chemical analysis and AF4-MALS-dRI. The following repeat unit structures were determined: -4)[α-l-Rhap-[4-P-2-Gro]]-(1-3)-α-d-Sug-[6-P-Cho]-(1-3)-β-l-Rhap-[2-OAc]-(1-4)-β-d-Glcp-(1-; 28F: Sug = Glcp, Mw: 540.5 kDa; 28A: Sug = GlcpNAc, Mw: 421.9 kDa; The correlation of CPS structures with biosynthesis showed that glycosyltransferase WciU in serotypes 28F and 28A had different sugar donor specificity toward α-d-Glcp and α-d-GlcNAcp, respectively. Furthermore, latex agglutination tests of de-OAc and de-PO 4 CPS were conducted to understand cross-reactions between serogroup 28 with factor antiserum 23d. Interestingly, the de-OAc 28F and 28A CPS can still weakly react with factor antiserum 23d, while de-PO 4 CPS did not react with factor antiserum 23d. This indicated that OAc group could affect the affinity and P-2-Gro was crucial for cross-reacting with factor antiserum 23d.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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