Autor: |
Pérez-Rodríguez F; Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Irapuato , Irapuato Gto, México., González-Prieto JM, Vera-Núñez JA; Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Irapuato , Irapuato Gto, México., Ruiz-Medrano R; Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Zacatenco , Ciudad de México, México., Peña-Cabriales JJ; Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Irapuato , Irapuato Gto, México., Ruiz-Herrera J; Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Irapuato , Irapuato Gto, México. |
Jazyk: |
angličtina |
Zdroj: |
Plant signaling & behavior [Plant Signal Behav] 2021 Feb 01; Vol. 16 (2), pp. 1855016. Date of Electronic Publication: 2020 Dec 23. |
DOI: |
10.1080/15592324.2020.1855016 |
Abstrakt: |
We have previously described that laboratory strains of Ustilago maydis , a fungal pathogen of maize and its ancestor teosinte, harbor an intracellular bacterium that enables the fungus to fix nitrogen. However, it is not clear whether other strains isolated from nature also harbor endosymbiotic bacteria, and whether these fix nitrogen for its host. In the present study, we isolated U. maydis strains from naturally infected maize. All the isolated strains harbored intracellular bacteria as determined by PCR amplification of the 16S rRNA gene, and some of them showed capacity to fix nitrogen. That these are truly bacterial endosymbionts were shown by the fact that, after thorough treatments with CuSO 4 followed by serial incubations with antibiotics, the aforementioned bacterial gene was still amplified in treated fungi. In all, these data support the notion that U. maydis -bacterium endosymbiosis is a general phenomenon in this species. |
Databáze: |
MEDLINE |
Externí odkaz: |
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