Self-Assembly of Solubilized Human Hair Keratins.

Autor: Lai HY; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.; Nanyang Environment & Water Research Institute (Environmental Chemistry and Materials Centre), Interdisciplinary Graduate Program, Nanyang Technological University, Singapore., Setyawati MI; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore., Ferhan AR; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore., Divakarla SK; The University of Sydney, Sydney Nano Institute, Faculty of Medicine and Health, Sydney Pharmacy School, Sydney, New South Wales 2006, Australia., Chua HM; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore., Cho NJ; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore., Chrzanowski W; The University of Sydney, Sydney Nano Institute, Faculty of Medicine and Health, Sydney Pharmacy School, Sydney, New South Wales 2006, Australia., Ng KW; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.; Nanyang Environment & Water Research Institute (Environmental Chemistry and Materials Centre), Interdisciplinary Graduate Program, Nanyang Technological University, Singapore.; Skin Research Institute of Singapore, Biomedical Science Institutes, Immunos, 8A Biomedical Grove, Singapore 138648, Singapore.; Department of Environmental Health, Harvard T.H. Chan School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115, United States.
Jazyk: angličtina
Zdroj: ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2021 Jan 11; Vol. 7 (1), pp. 83-89. Date of Electronic Publication: 2020 Dec 23.
DOI: 10.1021/acsbiomaterials.0c01507
Abstrakt: Human hair keratins have proven to be a viable biomaterial for diverse regenerative applications. However, the most significant characteristic of this material, the ability to self-assemble into nanoscale intermediate filaments, has not been exploited. Herein, we successfully demonstrated the induction of hair-extracted keratin self-assembly in vitro to form dense, homogeneous, and continuous nanofibrous networks. These networks remain hydrolytically stable in vitro for up to 5 days in complete cell culture media and are compatible with primary human dermal fibroblasts and keratinocytes. These results enhance the versatility of human hair keratins for applications where structured assembly is of benefit.
Databáze: MEDLINE