Acoel Single-Cell Transcriptomics: Cell Type Analysis of a Deep Branching Bilaterian.

Autor:	Duruz J; Department of Biology, Institute of Zoology, University of Fribourg, Fribourg, Switzerland., Kaltenrieder C; Department of Biology, Institute of Zoology, University of Fribourg, Fribourg, Switzerland., Ladurner P; Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, Innsbruck, Austria., Bruggmann R; Institute of Cell Biology, University of Bern, Bern, Switzerland.; Interfaculty Bioinformatics Unit, University of Bern, Bern, Switzerland., Martìnez P; Departament de Genètica, Universitat de Barcelona, Barcelona, Catalonia, Spain.; Institut Català de Recerca i Estudis Avancats (ICREA), Passeig de Lluís Companys, Barcelona, Spain., Sprecher SG; Department of Biology, Institute of Zoology, University of Fribourg, Fribourg, Switzerland.
Jazyk:	angličtina
Zdroj:	Molecular biology and evolution [Mol Biol Evol] 2021 May 04; Vol. 38 (5), pp. 1888-1904.
DOI:	10.1093/molbev/msaa333
Abstrakt:	Bilaterian animals display a wide variety of cell types, organized into defined anatomical structures and organ systems, which are mostly absent in prebilaterian animals. Xenacoelomorpha are an early-branching bilaterian phylum displaying an apparently relatively simple anatomical organization that have greatly diverged from other bilaterian clades. In this study, we use whole-body single-cell transcriptomics on the acoel Isodiametra pulchra to identify and characterize different cell types. Our analysis identifies the existence of ten major cell type categories in acoels all contributing to main biological functions of the organism: metabolism, locomotion and movements, behavior, defense, and development. Interestingly, although most cell clusters express core fate markers shared with other animal clades, we also describe a surprisingly large number of clade-specific marker genes, suggesting the emergence of clade-specific common molecular machineries functioning in distinct cell types. Together, these results provide novel insight into the evolution of bilaterian cell types and open the door to a better understanding of the origins of the bilaterian body plan and their constitutive cell types. (© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu