| Autor: |
Nifant'ev I; Chemistry Department, M.V. Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow, Russia.; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia.; Faculty of Chemistry, National Research University Higher School of Economics, 20 Miasnitskaya Str., 101000 Moscow, Russia., Shlyakhtin A; Chemistry Department, M.V. Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow, Russia., Komarov P; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia., Tavtorkin A; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia., Kananykhina E; Research Institute of Human Morphology, 3 Tsyurupy St., 117418 Moscow, Russia., Elchaninov A; National Medical Research Center for Obstetrics Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 4 Oparina Str., 117997 Moscow, Russia., Vishnyakova P; National Medical Research Center for Obstetrics Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 4 Oparina Str., 117997 Moscow, Russia., Fatkhudinov T; Research Institute of Human Morphology, 3 Tsyurupy St., 117418 Moscow, Russia.; Department of Histology, Cytology and Embryology, Peoples' Friendship University of Russia, Miklukho-Maklaya 6 Str., 117198 Moscow, Russia., Ivchenko P; Chemistry Department, M.V. Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow, Russia.; A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia. |
| Abstrakt: |
The control of surface bioadhesive properties of the subcutaneous implants is essential for the development of biosensors and controlled drug release devices. Poly(alkyl ethylene phosphate)-based (co)polymers are structurally versatile, biocompatible and biodegradable, and may be regarded as an alternative to poly(ethylene glycol) (PEG) copolymers in the creation of antiadhesive materials. The present work reports the synthesis of block copolymers of ε-caprolactone (εCL) and 2-ethoxy-1,3,2-dioxaphospholane-2-oxide (ethyl ethylene phosphate, EtOEP) with different content of EtOEP fragments, preparation of polymer films, and the results of the study of the impact of EtOEP/εCL ratio on the hydrophilicity (contact angle of wetting), hydrolytic stability, cytotoxicity, protein and cell adhesion, and cell proliferation using umbilical cord multipotent stem cells. It was found that the increase of EtOEP/εCL ratio results in increase of hydrophilicity of the polymer films with lowering of the protein and cell adhesion. MTT cytotoxicity test showed no significant deviations in toxicity of poly(εCL) and poly(εCL)- b -poly(EtOEP)-based films. The influence of the length of poly(EtOEP)chain in block-copolymers on fibrotic reactions was analyzed using subcutaneous implantation experiments (Wistar line rats), the increase of the width of the fibrous capsule correlated with higher EtOEP/εCL ratio. However, the copolymer-based film with highest content of polyphosphate had been subjected to faster degradation with a formation of developed contact surface of poly(εCL). The rate of the degradation of polyphosphate in vivo was significantly higher than the rate of the degradation of polyphosphate in vitro, which only confirms an objective value of in vivo experiments in the development of polymer materials for biomedical applications. |
