| Autor: |
Olivares E; Virulence Bactérienne Précoce UR7290, ITI Innovec, FMTS, Institut de Bactériologie, Université de Strasbourg, 3 Rue Koeberlé, 67000 Strasbourg, France.; BioFilm Pharma SAS, 317 Avenue Jean Jaurès, 69007 Lyon, France., Tasse J; BioFilm Pharma SAS, 317 Avenue Jean Jaurès, 69007 Lyon, France., Badel-Berchoux S; BioFilm Control SAS, Rue Emile Duclaux, 63360 Saint Beauzire, France., Provot C; BioFilm Pharma SAS, 317 Avenue Jean Jaurès, 69007 Lyon, France.; BioFilm Control SAS, Rue Emile Duclaux, 63360 Saint Beauzire, France., Prévost G; Virulence Bactérienne Précoce UR7290, ITI Innovec, FMTS, Institut de Bactériologie, Université de Strasbourg, 3 Rue Koeberlé, 67000 Strasbourg, France., Bernardi T; BioFilm Pharma SAS, 317 Avenue Jean Jaurès, 69007 Lyon, France.; BioFilm Control SAS, Rue Emile Duclaux, 63360 Saint Beauzire, France. |
| Abstrakt: |
Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test ® (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of biofilm-producer strains among P. aeruginosa samples isolated from expectorations of cystic fibrosis (CF) patients. Despite a majority of spontaneous adherent isolates, few strains remained planktonic after 5 h of incubation. Their analysis by an adapted protocol of the cBRT revealed an induction of the biofilm early formation by sub-inhibitory doses of β-lactams. Microscopic observations of bacterial cultures stained with Syto 9/Propidium Iodide (PI) confirmed the ability of antimicrobials to increase either the bacterial biomass or the biovolume occupied by induced sessile cells. Finally, the cBRT and its derivatives enabled to highlight in a few hours the potential inducer property of antibiotics on bacterial adhesion. This phenomenon should be considered carefully in the context of CF since patients are constantly under fluctuating antimicrobial treatments. To conclude, assays derived from the Biofilm Ring Test ® (BRT) device, not only define efficient doses preventing biofilm formation, but could be useful for the antimicrobial selection in CF, to avoid inducer molecules of the early biofilm initiation. |
