Biochemical characterization of two chitinases from Bacillus cereus sensu lato B25 with antifungal activity against Fusarium verticillioides P03.

Autor:	Morales-Ruiz E; Departamento de Biotecnología Agrícola, CIIDIR Unidad Sinaloa, Instituto Politécnico Nacional, Boulevard Juan de Dios Bátiz Paredes 250, 81101, Col. San Joachin, Guasave, Sinaloa, Mexico., Priego-Rivera R; Departamento de Biotecnología Agrícola, CIIDIR Unidad Sinaloa, Instituto Politécnico Nacional, Boulevard Juan de Dios Bátiz Paredes 250, 81101, Col. San Joachin, Guasave, Sinaloa, Mexico., Figueroa-López AM; Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Col. Centro, 85000, Ciudad Obregón, Sonora, Mexico., Cazares-Álvarez JE; Departamento de Biotecnología Agrícola, CIIDIR Unidad Sinaloa, Instituto Politécnico Nacional, Boulevard Juan de Dios Bátiz Paredes 250, 81101, Col. San Joachin, Guasave, Sinaloa, Mexico., Maldonado-Mendoza IE; Departamento de Biotecnología Agrícola, CIIDIR Unidad Sinaloa, Instituto Politécnico Nacional, Boulevard Juan de Dios Bátiz Paredes 250, 81101, Col. San Joachin, Guasave, Sinaloa, Mexico.
Jazyk:	angličtina
Zdroj:	FEMS microbiology letters [FEMS Microbiol Lett] 2021 Feb 04; Vol. 368 (2).
DOI:	10.1093/femsle/fnaa218
Abstrakt:	Bacterial chitinases are a subject of intense scientific research due to their biotechnological applications, particularly their use as biological pesticides against phytopathogenic fungi as a green alternative to avoid the use of synthetic pesticides. Bacillus cereus sensu lato B25 is a rhizospheric bacterium that is a proven antagonist of Fusarium verticillioides, a major fungal pathogen of maize. This bacterium produces two chitinases that degrade the fungal cell wall and inhibit its growth. In this work, we used a heterologous expression system to purify both enzymes to investigate their biochemical traits in terms of Km, Vmax, optimal pH and temperature. ChiA and ChiB work as exochitinases, but ChiB exhibited a dual substrate activity and it is also an endochitinase. In this work, the direct addition of these chitinases inhibited fungal conidial germination and therefore they may play a major role in the antagonism against F. verticillioides. (© The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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