Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging.

Autor: Bottes S; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Jaeger BN; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Pilz GA; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Jörg DJ; Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK.; The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.; Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge, Cambridge, UK., Cole JD; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Kruse M; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Harris L; Neural Stem Cell Biology Laboratory, The Francis Crick Institute, London, UK., Korobeynyk VI; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Mallona I; Institute of Molecular Life Sciences and SIB Swiss Institute of Bioinformatics, University of Zurich, Zurich, Switzerland.; Department of Molecular Mechanisms of Disease, University of Zurich, Zurich, Switzerland., Helmchen F; Laboratory of Neural Circuit Dynamics, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland., Guillemot F; Neural Stem Cell Biology Laboratory, The Francis Crick Institute, London, UK., Simons BD; Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK.; The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.; Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge, Cambridge, UK., Jessberger S; Laboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland. jessberger@hifo.uzh.ch.
Jazyk: angličtina
Zdroj: Nature neuroscience [Nat Neurosci] 2021 Feb; Vol. 24 (2), pp. 225-233. Date of Electronic Publication: 2020 Dec 21.
DOI: 10.1038/s41593-020-00759-4
Abstrakt: Neural stem cells (NSCs) generate neurons throughout life in the mammalian hippocampus. However, the potential for long-term self-renewal of individual NSCs within the adult brain remains unclear. We used two-photon microscopy and followed NSCs that were genetically labeled through conditional recombination driven by the regulatory elements of the stem cell-expressed genes GLI family zinc finger 1 (Gli1) or achaete-scute homolog 1 (Ascl1). Through intravital imaging of NSCs and their progeny, we identify a population of Gli1-targeted NSCs showing long-term self-renewal in the adult hippocampus. In contrast, once activated, Ascl1-targeted NSCs undergo limited proliferative activity before they become exhausted. Using single-cell RNA sequencing, we show that Gli1- and Ascl1-targeted cells have highly similar yet distinct transcriptional profiles, supporting the existence of heterogeneous NSC populations with diverse behavioral properties. Thus, we here identify long-term self-renewing NSCs that contribute to the generation of new neurons in the adult hippocampus.
Databáze: MEDLINE
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