| Autor: |
Riyanti; Institute for Insect Biotechnology, Justus-Liebig-University of Giessen, 35392 Giessen, Germany.; Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto 53122, Indonesia.; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, 35392 Giessen, Germany., Marner M; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, 35392 Giessen, Germany., Hartwig C; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, 35392 Giessen, Germany., Patras MA; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, 35392 Giessen, Germany., Wodi SIM; Department of Fisheries and Marine Science, Politeknik Negeri Nusa Utara, Tahuna Sangihe Islands, North Sulawesi 95812, Indonesia., Rieuwpassa FJ; Department of Fisheries and Marine Science, Politeknik Negeri Nusa Utara, Tahuna Sangihe Islands, North Sulawesi 95812, Indonesia., Ijong FG; Department of Fisheries and Marine Science, Politeknik Negeri Nusa Utara, Tahuna Sangihe Islands, North Sulawesi 95812, Indonesia.; Faculty of Fisheries and Marine Science, Sam Ratulangi University, Manado 95115, Indonesia., Balansa W; Department of Fisheries and Marine Science, Politeknik Negeri Nusa Utara, Tahuna Sangihe Islands, North Sulawesi 95812, Indonesia., Schäberle TF; Institute for Insect Biotechnology, Justus-Liebig-University of Giessen, 35392 Giessen, Germany.; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, 35392 Giessen, Germany.; German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, 35392 Giessen, Germany. |
| Abstrakt: |
The discovery of novel natural products (NPs) that will serve as lead structures has to be an ongoing effort to fill the respective development pipelines. However, identification of NPs, which possess a potential for application in e.g., the pharma or agro sector, must be as cost effective and fast as possible. Furthermore, the amount of sample available for initial testing is usually very limited, not least because of the fact that the impact on the environment, i.e., the sampled biosystem, should be kept minimal. Here, our pipeline SeaPEPR is described, in which a primary bioactivity screening of crude extracts is combined with the analysis of their metabolic fingerprint. This enabled prioritization of samples for subsequent microfractionation and dereplication of the active compounds early in the workflow. As a case study, 76 marine sponge-derived extracts were screened against a microbial screening panel. Thereunder, human pathogenic bacteria ( Escherichia coli ATCC35218 and Staphylococcus aureus ATCC33592) and yeast ( Candida albicans FH2173), as well as the phytopathogenic fungus Septoria tritici MUCL45407. Overall, nine extracts revealed activity against at least one test organism. Metabolic fingerprinting enabled assigning four active extracts into one metabolic group; therefore, one representative was selected for subsequent microfractionation. Dereplication of the active fractions showed a new dibrominated aplysinopsin and a hypothetical chromazonarol stereoisomer derivative. Furthermore, inhibitory activity against the common plant pest Septoria tritici was discovered for NPs of marine origin. |
