Silicea terra and Zincum metallicum Modulate the Activity of Macrophages Challenged with BCG In Vitro.

Autor: Pinto SAG; Research Center, Graduation Program in Environmental and Experimental Pathology, Universidade Paulista-UNIP, São Paulo, Brazil., Nagai MYO; Research Center, Graduation Program in Environmental and Experimental Pathology, Universidade Paulista-UNIP, São Paulo, Brazil., Alvares-Saraiva A; Research Center, Graduation Program in Environmental and Experimental Pathology, Universidade Paulista-UNIP, São Paulo, Brazil., Peres GB; Research Center, Graduation Program in Environmental and Experimental Pathology, Universidade Paulista-UNIP, São Paulo, Brazil., Waisse S; Pontificia Universidade Católica de São Paulo, Graduation Program in History of Science, São Paulo, Brazil., Perez EC; Research Center, Graduation Program in Environmental and Experimental Pathology, Universidade Paulista-UNIP, São Paulo, Brazil., Bonamin LV; Research Center, Graduation Program in Environmental and Experimental Pathology, Universidade Paulista-UNIP, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Homeopathy : the journal of the Faculty of Homeopathy [Homeopathy] 2021 Feb; Vol. 110 (1), pp. 52-61. Date of Electronic Publication: 2020 Dec 21.
DOI: 10.1055/s-0040-1716367
Abstrakt: Background:  The homeopathic medicines Silicea terra ( Sil ) and Zincum metallicum ( Zinc ) modulate macrophage activity and were assessed in an experimental study in-vitro for their effects on macrophage-BCG (Bacillus Calmette-Guérin) interaction.
Methods:  RAW 264.7 macrophages were infected with BCG, treated with different potencies of Sil and Zinc (6cH, 30cH and 200cH) or vehicle, and assessed 24 and 48 h later for bacilli internalization, hydrogen peroxide (H 2 O 2 ) and cytokine production, and lysosomal activity.
Results:  Treatment with vehicle was associated with non-specific inhibition of H 2 O 2 production to the levels exhibited by uninfected macrophages. Sil 200cH induced significant reduction of H 2 O 2 production ( p  < 0.001) compared with the vehicle and all other treatments, as well as higher lysosomal activity ( p  ≤ 0.001) and increased IL-10 production ( p  ≤ 0.05). Such effects were considered specific for this remedy and potency. The number of internalized bacilli was inversely proportional to Zinc potencies, with statistically significant interaction between dilution and treatment ( p  = 0.003). Such linear-like behavior was not observed for Sil dilutions: peak internalization occurred with the 30cH dilution, accompanied by cellular degeneration, and IL-6 and IL-10 increased ( p  ≤ 0.05) only in the cells treated with Sil 6cH.
Conclusion:   Sil and Zinc presented different patterns of potency-dependent effect on macrophage activity. Bacterial digestion and a balanced IL-6/IL-10 production were related to Sil 6cH, though reduced oxidative stress with increased lysosomal activity was related to Sil 200cH. Degenerative effects were exclusively related to Sil 30cH, and potency-dependent phagocytosis was related only to Zinc .
Competing Interests: Dr. Bonamin reports grants from UNIP.
(Faculty of Homeopathy. This article is published by Thieme.)
Databáze: MEDLINE
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