Frequent asymptomatic infection with tobacco ringspot virus on melon fruit.

Autor: Tabara M; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan; Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan. Electronic address: midori.tabara@gmail.com., Nagashima Y; Vegitable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA., He K; Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, 77843, USA., Qian X; Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, 77843, USA; Department of Computer and Science Engineering, Texas A&M University, College Station, TX, 77843, USA; TEES-AgriLife Center for Bioinformatics & Genomic Systems Engineering, Texas A&M University, College Station, TX, 77843, USA., Crosby KM; Vegitable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA., Jifon J; Vegitable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA., Jayaprakasha GK; Vegitable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA., Patil B; Vegitable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA; Department of Nutrition and Food Science, Texas A&M University, College Station, TX, 77843, USA., Koiwa H; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan; Vegitable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA., Takahashi H; Graduate School of Agricultural Science, Tohoku University, 468-1, Aramaki-Aza-Aoba, Sendai 980-0845, Japan., Fukuhara T; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan; Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan.
Jazyk: angličtina
Zdroj: Virus research [Virus Res] 2021 Feb; Vol. 293, pp. 198266. Date of Electronic Publication: 2020 Dec 24.
DOI: 10.1016/j.virusres.2020.198266
Abstrakt: Melon is one of the most popular fruits worldwide and has been bred into various cultivars. RNA-sequencing using healthy melon fruit was performed to determine differences in gene expression among cultivars. Unexpected RNA-seq results revealed that viruses asymptomatically infected fruits at a high frequency (16 of 21 fruits examined were infected) and that viral transcripts highly accumulated in comparison with host transcripts (15 %-75 % of total reads). Their nucleotide sequences and phylogenetic analyses indicated that more than 10 novel isolates of tobacco ringspot virus (TRSV) were found in melon fruits. Asymptomatic infection with TRSV on melon fruits was confirmed by both immunoblot and RT-PCR analyses. Numerous isolates of TRSV generated and maintained in melon fields, and this is likely due to their asymptomatic infections. This TRSV melon isolate infected Nicotiana benthamiana plants with stunting and yellowing symptoms. This is the first report of frequent and asymptomatic infection of TRSV in consumable melon fruits.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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