Small Mammals as Carriers/Hosts of Leptospira spp . in the Western Amazon Forest.

Autor:	Medeiros LDS; Laboratório de Microbiologia e Imunologia Veterinária, Universidade Federal do Acre, Rio Branco, Brazil., Braga Domingos SC; Laboratório de Microbiologia e Imunologia Veterinária, Universidade Federal do Acre, Rio Branco, Brazil., Azevedo MIND; Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Niterói, Brazil., Peruquetti RC; Laboratório de Microbiologia e Imunologia Veterinária, Universidade Federal do Acre, Rio Branco, Brazil., de Albuquerque NF; Laboratório de Microbiologia e Imunologia Veterinária, Universidade Federal do Acre, Rio Branco, Brazil., D'Andrea PS; Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, IOC, Fiocruz, Rio de Janeiro, Brazil., Botelho ALM; Instituto Federal de Educação, Ciência e Tecnologia do Acre, Rio Branco, Brazil., Crisóstomo CF; Instituto Federal de Educação, Ciência e Tecnologia do Acre, Rio Branco, Brazil., Vieira AS; Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Niterói, Brazil., Martins G; Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Niterói, Brazil., Teixeira BR; Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, IOC, Fiocruz, Rio de Janeiro, Brazil., Carvalho-Costa FA; Laboratório de Epidemiologia e Sistemática Molecular, IOC, Fiocruz, Rio de Janeiro, Brazil., Lilenbaum W; Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense, Niterói, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2020 Dec 02; Vol. 7, pp. 569004. Date of Electronic Publication: 2020 Dec 02 (Print Publication: 2020).
DOI:	10.3389/fvets.2020.569004
Abstrakt:	Leptospira is a bacteria that causes leptospirosis and is transmitted through water, soil, or mud that is contaminated by the urine of infected animals. Although it is mainly associated with the urban environment, Leptospires also circulate in rural and wild environments. This study aimed to investigate the role of small mammals in leptospirosis epidemiology in the western Amazon, Brazil. In total, 103 animals from 23 species belonging to the orders Didelphimorphia and Rodentia were captured. Blood, kidney, and urine samples were collected and Microscopic Agglutination Test (MAT), lip L32 PCR, sec Y sequencing, and culturing were conducted. MAT was reactive on 1/15 sera, and no bacterial isolate was obtained. PCR yielded 44.7% positive samples from 16 species. Twenty samples were genetically characterized and identified as L. interrogans ( n = 12), L. noguchii ( n = 4), and L. santarosai ( n = 4). No statistical association was found between the prevalence of infection by Leptospira spp. in small mammals within carrier/hosts species, orders, study area, and forest strata. Our results indicate a high prevalence of pathogenic Leptospira spp. in several rodent and marsupial species and report the first evidence of Leptospira spp. carrier/hosts in the Brazilian Western Amazon. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2020 Medeiros, Braga Domingos, Azevedo, Peruquetti, de Albuquerque, D'Andrea, Botelho, Crisóstomo, Vieira, Martins, Teixeira, Carvalho-Costa and Lilenbaum.)
Databáze:	MEDLINE
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