| Autor: |
Ortiz-Andrade R; Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Merida 97069, Yucatan, Mexico., Araujo-León JA; Grupo de Investigación en Química Analítica y Ambiental, Facultad de Química, Universidad Autónoma de Yucatán, Merida 97069, Yucatan, Mexico.; Facultad de Química, Unidad Académica de Ciencias y Tecnología de la UNAM en Yucatán, Sierra Papacal 97302, Yucatan, Mexico., Sánchez-Recillas A; Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Merida 97069, Yucatan, Mexico., Navarrete-Vazquez G; Laboratorio de Química Farmacéutica, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, Mexico., González-Sánchez AA; Facultad de Ingeniería, Universidad Autónoma de Yucatán, Merida 92203, Yucatan, Mexico., Hidalgo-Figueroa S; Research, Innovation and Development Consortium for Arid Areas, Potosino Institute of Scientific and Technological Research, San Luis Potosi 78216, San Luis Potosi, Mexico., Alonso-Castro ÁJ; División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato 36050, Guanajuato, Mexico., Aranda-González I; Licenciatura en Nutrición, Facultad de Medicina, Universidad Autónoma de Yucatán, Merida 97000, Yucatan, Mexico., Hernández-Núñez E; Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Istituto Politécnico Nacional-Unidad Mérida, Merida 97205, Yucatan, Mexico., Coral-Martínez TI; Grupo de Investigación en Química Analítica y Ambiental, Facultad de Química, Universidad Autónoma de Yucatán, Merida 97069, Yucatan, Mexico., Sánchez-Salgado JC; Hypermedic MX, Hacienda Santa Cecilia 97, Cafetales I, Coyoacán 04930, Mexico City, Mexico., Yáñez-Pérez V; Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Merida 97069, Yucatan, Mexico.; Bioterio de la Escuela de Medicina, Universidad Anáhuac-Mayab, Merida 97310, Yucatan, Mexico., Lucio-Garcia MA; Grupo de Investigación en Química Analítica y Ambiental, Facultad de Química, Universidad Autónoma de Yucatán, Merida 97069, Yucatan, Mexico. |
| Abstrakt: |
Many studies describe different pharmacological effects of flavonoids on experimental animals and humans. Nevertheless, few ones are confirming the safety of these compounds for therapeutic purposes. This study aimed to investigate the preclinical safety of naringenin, naringin, hesperidin, and quercetin by in vivo, in vitro, and in silico approaches. For this, an MTT-based cytotoxicity assay in VERO and MDCK cell lines was performed. In addition, acute toxicity was evaluated on Wistar rats by OECD Guidelines for the Testing of Chemicals (Test No. 423: Acute Oral Toxicity-Class Method). Furthermore, we used the ACD/Tox Suite to predict toxicological parameters such as hERG channel blockade, CYP450 inhibition, and acute toxicity in animals. The results showed that quercetin was slightly more cytotoxic on cell lines (IC 50 of 219.44 ± 7.22 mM and 465.41 ± 7.44 mM, respectively) than the other citroflavonoids. All flavonoids exhibited an LD 50 value > 2000 mg/kg, which classifies them as low-risk substances as OECD guidelines established. Similarly, predicted LD 50 was LD 50 > 300 to 2000 mg/kg for all flavonoids as acute toxicity assay estimated. Data suggests that all these flavonoids did not show significant toxicological effects, and they were classified as low-risk, useful substances for drug development. |
