The immunophilin Zonda controls regulated exocytosis in endocrine and exocrine tissues.

Autor: de la Riva-Carrasco R; Laboratorio de Genética y Fisiología Molecular, Fundación Instituto Leloir, Buenos Aires, Argentina., Perez-Pandolfo S; Laboratorio de Genética y Fisiología Molecular, Fundación Instituto Leloir, Buenos Aires, Argentina., Suárez Freire S; Laboratorio de Genética y Fisiología Molecular, Fundación Instituto Leloir, Buenos Aires, Argentina., Romero NM; Université Côte d'Azur, INRA, CNRS, Institut Sophia Agrobiotech, Sophia Antipolis, France., Bhujabal Z; Molecular Cancer Research Group, Department of Medical Biology, University of Tromsø-The Arctic University of Norway, Tromsø, Norway., Johansen T; Molecular Cancer Research Group, Department of Medical Biology, University of Tromsø-The Arctic University of Norway, Tromsø, Norway., Wappner P; Laboratorio de Genética y Fisiología Molecular, Fundación Instituto Leloir, Buenos Aires, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales-Universidad de Buenos Aires, Buenos Aires, Argentina., Melani M; Laboratorio de Genética y Fisiología Molecular, Fundación Instituto Leloir, Buenos Aires, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Traffic (Copenhagen, Denmark) [Traffic] 2021 Apr; Vol. 22 (4), pp. 111-122. Date of Electronic Publication: 2021 Jan 25.
DOI: 10.1111/tra.12777
Abstrakt: Exocytosis is a fundamental process in physiology, that ensures communication between cells, organs and even organisms. Hormones, neuropeptides and antibodies, among other cargoes are packed in exocytic vesicles that need to reach and fuse with the plasma membrane to release their content to the extracellular milieu. Hundreds of proteins participate in this process and several others in its regulation. We report here a novel component of the exocytic machinery, the Drosophila transmembrane immunophilin Zonda (Zda), previously found to participate in autophagy. Zda is highly expressed in secretory tissues, and regulates exocytosis in at least three of them: the ring gland, insulin-producing cells and the salivary gland. Using the salivary gland as a model system, we found that Zda is required at final steps of the exocytic process for fusion of secretory granules to the plasma membrane. In a genetic screen we identified the small GTPase RalA as a crucial regulator of secretory granule exocytosis that is required, similarly to Zda, for fusion between the secretory granule and the plasma membrane.
(© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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