DNA aptamer-based rolling circle amplification product as a novel immunological adjuvant.

Autor: Al-Ogaili AS; Department of Cell and Molecular Biology, University of Arkansas, Fayetteville, AR, 72701, USA.; Department of Medical Laboratory Techniques, Kut Tech Institute, Middle Technical University, Baghdad, Iraq., Liyanage R; Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.; Statewide Mass Spectrometry Facility, Fayetteville, AR, 72701, USA., Lay JO Jr; Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.; Statewide Mass Spectrometry Facility, Fayetteville, AR, 72701, USA., Jiang T; Department of Poultry Science, University of Arkansas, Fayetteville, AR, 72701, USA., Vuong CN; Department of Poultry Science, University of Arkansas, Fayetteville, AR, 72701, USA., Agrawal S; Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA., Kumar TKS; Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA., Berghman LR; Department of Poultry Science and Pathobiology, Texas A&M University, College Station, TX, 77843, USA., Hargis BM; Department of Cell and Molecular Biology, University of Arkansas, Fayetteville, AR, 72701, USA.; Department of Poultry Science, University of Arkansas, Fayetteville, AR, 72701, USA., Kwon YM; Department of Cell and Molecular Biology, University of Arkansas, Fayetteville, AR, 72701, USA. ykwon@uark.edu.; Department of Poultry Science, University of Arkansas, Fayetteville, AR, 72701, USA. ykwon@uark.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Dec 17; Vol. 10 (1), pp. 22282. Date of Electronic Publication: 2020 Dec 17.
DOI: 10.1038/s41598-020-79420-w
Abstrakt: Several agonists to CD40 have shown to induce acquired immune responses. Here, we developed and evaluated the rolling circle amplification (RCA) products that are based on anti-CD40 DNA aptamers as a novel vaccine adjuvant. First, we developed DNA aptamers with specific binding affinity to chicken CD40 extra domain (chCD40ED). Next, we prepared the RCA products that consist of these aptamers to increase the spanning space and overall binding affinity to chCD40ED. Using 8 DNA aptamer candidates, 4 aptamer-based RCA products (aptamer RCAs) were generated, each consisting of two distinct aptamers. We demonstrated that all 4 aptamer RCAs significantly induced the signal transduction in chicken HD11 macrophage cell line (p < 0.05). Finally, we conjugated one of the aptamer RCAs (Aptamer RCA II) to M2e epitope peptide of influenza virus as a model hapten, and the immune complex was injected to chickens. Aptamer RCA II stimulated anti-M2e IgG antibody production to the level significantly higher as compared to the control (M2e epitope alone; p < 0.05). The results of our work suggest that aptamer RCA is a novel platform to boost the efficacy of vaccines, which might find broad applications to other antigens beyond M2e epitope evaluated in this study using chicken infection model.
Databáze: MEDLINE
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